The Protective Effect Of Ulinastatin Combined With Mild Hypothermia On The Barrier Function Of Intestinal Mucous Membrane Of Rat After The Cardiopulmonary Resuscitation

ObjectiveThe protective effect and possible mechanism of ulinastatin combined with mild hypothermia on the barrier function of intestinal mucous membrane of rat after cardiopulmonary resuscitation were preliminary discussed.MethodsA total of25SD rats were selected to create the cardiac arrest model for the cardiopulmonary resuscitation by suffocation method. They were randomly divided into sham operation group, control group, mild hypothermia group, ulinastatin group and ulinastatin combined with mild hypothermia group (5in each group). The sham operation group was only performed with arteriovenous catheterization and endotracheal intubation. The rat was executed and the intestinal tissue specimens were withdrawn after6hours after the operation. The intestinal tissue specimens were withdrawn6hours after the cardiopulmonary resuscitation in other groups. And the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. The damage situation of small intestinal tissue were compared between each group observed with the transmission electron microscopy.Results1. Compared with the sham operation group, the MDA protein content of the control group increased and SOD activity decreased with significant difference (P<0.05).2. Compared with the control group, the MDA protein contents of mild hypothermia group and ulinastatin group decreased, and SOD activity increased with significant difference (P<0.05).3.Compared with the mild hypothermia group and ulinastatin group, the MDA protein content further decreased and SOD activity further increased in the ulinastatin combined with mild hypothermia group with significant difference (P<0.05). However, the MDA content and SOD activity of the mild hypothermia group were not significantly different from the ulinastatin group (P>0.05).4. The intestinal mucosa of all the groups were found to be damaged after recovery under the electron microscope. The epithelial tight junction was opened and shortened in different degree with different degrees of the infiltration of inflammatory cells and cell nucleus pycnosis. However compared with the control group, the damage condition of the intestinal mucosa barrier of the mild hypothermia group and ulinastatin group, especially the ulinastatin combined with mild hypothermia group were reduced significantly.ConclusionsUlinastatin and mild hypothermia can play a role of protection of intestinal mucosa after recovery by inhibiting the oxidative stress reaction of small intestine tissues in rats after cardiopulmonary resuscitation. The combination effect appears as the stronger synergy or additive effect on the protection of the intestinal mucosa barrier.