Expression And Clinic Significance Of MMP-9and RUNX3in Duodenal Adenocarcinoma

ObjectiveTo make clear the roles which MMP-9and RUNX3played in duodenaladenocarcinoma and their relationship, we intended to detect the expression of them andanalyzed the relationship among them. we hope to know the relationship between theirexpression and clinic significance in duodenal adenocarcinoma. The survival analysis toexplore the MMP-9and RUNX3with duodenal adenocarcinoma prognosis thatcombination of follow-up data, estimate the relationship between MMP-9、RUNX3andprognosis.Methodschoose60cases of duodenal adenocarcinoma and40cases of paracancer tissues.SPimmunohistochemical method was applied to detect the expression of MMP-9andRUNX3in that cases. Analyse the relationship between expression of MMP-9andRUNX3and the development、clinicalpatholog and prognosis of colorectal cancer. Alldata were processed by SPSS17.0.Results(1) Expression of MMP-9The positive expression rate of MMP-9in duodenaladenocarcinoma was70.0%, which was significantly higher than that in normal tissuessamples27.5%,(χ2=17.4, P<0.01); The expression of MMP-9in duodenaladenocarcinoma were associated with the depth of infiltration and differentiation andlymph node metastasis but had nothing to do with patients, sex, tumor size,initial occurring position. MMP-9positive expression in invaded mucous membrane andmuscle was lower than that invaded tunica externa and out of tunicaexterna(P=0.017).MMP-9positive expression in poorly differentiated cancer type washigher than that in moderately and well differentiated type(P=0.029); The expressionrate in positive lymph nodes was higher than that of negative lymph nodes(P=0.007);.(2)Expression of RUNX3The positive expression rate of Runx3protein in duodenaladenocarcinoma was23.3%,which was significantly lower than that in normal tissuessamples77.5%,(χ~2=28.4, P<0.01). The expression of RUNX3in duodenaladenocarcinoma were associated with differentiation and lymph node metastasis but hadnothing to do with other clinicalpatholog. RUNX3positive expression in poorlydifferentiated cancer type was lower than that in moderately and well differentiatedtype(P=0.001); The expression rate in positive lymph nodes was lower than that ofnegative lymph nodes(P=0.033)(3) Expression and prognosis of MMP-9andRUNX3In the MMP-9positive expression group,1year、3year、5year survival ratewere respectively71.0%，36.9%，7.4%; while in negative group,1year、3year、5yearsurvival rate were respectively88.1%、49.0%、28.0%, the difference was significant（χ2=8.027，P=0.005）. In the RUNX3positive expression group,1year survival rate was85.7%、3year survival rate was68.6%,5year survival rate was34.3%; while in negativegroup,1year、3year、5year survival rate were respectively73.6%,32.5%,9.8%, thedifference was significan（tχ~2=8.491，P=0.004）.(4) Expression correlation of MMP-9and RUNX3In the42positive expressionMMP-9cancer cases, there were36casesthat negative expression of RUNX3; The negative expression rate was85.7%。In the18negative expressionMMP-9cancer cases, there were8cases that positive expression ofRUNX3; The positive expression rate was44.4%.Classified variable correlationanalysis was applied to indicate that there was significantly positive correlation betweenexpression of MMP-9and RUNX3(χ~2=6.406, P=0.011, r=0.311). Conclusions(1) MMP-9participated in development and metastasis of duodenal adenocarcinoma；MMP-9can be used as an important index to evaluate the invasion of duodenaladenocarcinoma. Through the principle of MMP-9promoting tumor can provide a newway for clinical treatment of duodenal adenocarcinoma.(2) RUNX3not only attended the duodenal adenocarcinoma but also participate in thedevelopment of duodenal adenocarcinoma.(3)Research RUNX3protein in duodenal adenocarcinoma added the blank of RUNX3in the digestive system, more prove the close relationship between RUNX3andgastrointestinal tumors.(4) The expression of MMP-9could judge prognosis of patients with duodenaladenocarcinoma. The expression of RUNX3could judge prognosis of patients withduodenal adenocarcinoma.(5) MMP-9may play roles in duodenal adenocarcinoma through interacting RUNX3.