Expression And Clinicopathological Correlation Of MiR-711in Different Human Gastric Cancer Cells And Tissues

Objective: To investigate the expression and effect of miR-711in differenthuman gastric cancer cells and tissues, then we explore the clinical value ofapplication preliminarily.Methods: We cultured four human gastric cancer cells of different degree ofdifferentiation:SGC-7901,BGC-823,AGS,MGC-803, and immortalized cells ofnormal gastric mucosa:GES-1. We collected28cases of gastric cancer patientsafter surgery to remove cancerous tissues and normal tissues. Detected express-ion levels of miR-711in gastric cancer cells and tissues by qRT-PCR, thenwe compared the expression levels of difference and analyzed ite correlation ofclinicopathology.Results: Detected expression levels of miR-711by qRT-PCR, it showed thatall human gastric cancer cells’ expression levels of miR-711were less thanexpression levels in the GES-1cells（P<0.05）. MiR-711expression in variouscells showed that GES-1cells were more than SGC-7901cells,and SGC-7901cells were more than BGC-823cells, AGS cells,MGC-803cells（P<0.05）.MiR-711expression level of BGC-823, AGS, MGC-803gastric cancer cells w-ere no significant difference（P>0.05）.Relative to normal tissue, miR-711expre-ssion level was significantly lower in gastric cancer tissues (P <0.05). Its expre-ssion level was significantly related to degree of differentiation, stage, lymphnode metastasis（P <0.05）, and unrelated to age, gender, tumor size（P>0.05）.Conclusion:MiR-711showed the low-expression in gastric cancer cells andtissues. It may be significantly related to the gastric cancer,because of iteunnormal expression...