The Effects Of Huashi Liquid On The Expression Of Proteins In Gastrointestinal Tract Of Rats With Dampness Retention Syndrome

BackgroundExogenou pathogen with characteristic of heavy and turbid,sticky andstagnant,descending is named dampness pathogen. Dampness RetentionSyndrome(DRS) refers to the obstruction of dampness in the middleenergizer(spleen and stomach) and has syndrom of oppression in thechest,heaviness of the body, lassitude, abdominal distention and anorexia,turbid and greasy coating on the tongue. In moder time, DRS-induceddisease becames more and more generally for the bad habit formation in thelife along with livng and working rhythm accelerating.Bad eat habit,coldfood,greasy food and so on can damage yang-qi of spleen and stomach andbe easy to stagnate qi movement.Otherwise,depress emotion can stagnateliver-qi that invades the spleen (earth).It can be another reason for thegeneration of dampness in vivo.DRS can occur as long as the obstruction ofdampness in the middle energizer(spleen and stomach). Furthermore, theetiopathogenesis of diseases such as gastrointestinal disease,Hepatobiliary diseases,Viral influenza,Hypertension and Diabetes are related to water, wetand phlegm-atic fluid.Some of these diseases display the DRS-pathogenesis.However, the curative effects of Western medicine in DRS-relation diseaseare not satisfied.Therefore,investigation to DRS-relation disease from theangle of TCM may has a very important clinical value.ObjectTo investigate the effects of HuaShi Liquid（HSL）on DRS-induceddysfunction of the gastrointestinal digestion and absorption,damage ofgastrointestinal barrier and metabolic disorder of liquid for providing muchmore experimental evidences to the effective and safe Chinese compoundrecipe (HSL),combinating with pro-experimental result.At the same time,the idea in study DRS can be used for the further research.The study will beoperated in the gene and protein level,that may act as experimental basis forthe latter cellule signal transduction and proteomics research.MethodsForty SD rats were randomly divided into5groups (Control，DRSgroup and three treatment groups (DRS+HSL),8rats in eachgroup．DRS was induced in all rats，except the control group，by modifiedenviromental and fatigued method for6days． After DRS model wasformed，control and DRS groups were lavaged with saline(NS). Rats intreatment groups were exposed to HSL by lavaged at different doses at4g/kg，8g/kg and16g/kg respectively for8days．Then the contents of D-xylose in serum were detected with colorimetric method,the content ofGhrelin in serum were deteced with double-antibody sandwichenzyme-linked immunosorbent assay(ELISA),the expression of AQP4oncolon mucosa and EGFR on gastric mucosa were all determined byimmunohistochemistry and the expression of AQP4mRNA on colonmucosa and ZO-1mRNA on ileum mucosa were detected by RT-PCR.Results1.The gastrointestinal digestion and absorption function: Rats in modelgroups exhibit typical dampness retention syndrom, with signs of poorappetite, loose stool hyperaemia, edema, hemorrhagic spots and sludgedblood in the gastric mucosa compared to control. The content of D-xylose（P<0.01）and Ghrelin（P<0.05）in serumdecreased obviously. Conversely,exposure of DRS rats to different doses of HSL resulted in increase in thecontent of D-xylose（P<0.01）and Ghrelin（P<0.05）. It’s suggesting thatHSL could repair dysfunction of gastrointestinal digestion and absorptionthat maybe related to increasing D-xylose and Ghrelin in serum.2.Liquid metabolism: The expression of AQP4in colon musocasignificantly decreased in DRS group compared to control group（P<0.05）.Conversely, exposure of DRS rats to different doses of HSLresulted in increase in the expression in AQP4in the colon mucosa （P<0.05versus DRS group）. It implts that HSL could have the therapeutical effect onDRS-induced diarrhoea, increasing expression of AQP4may play an important role.3.Defense function of Intestinal tract: The expression of ZO-1mRNAon ileum mucosa significantly decreased in DRS group compared to controlgroup（P<0.05）.Conversely, exposure of DRS rats to different doses of HSLresulted in increase in expression of ZO-1mRNA in the ileum mucosa（P<0.05versus DRS group）. HSL could have the protective effect onDRS-induced intestinal mucosal injury, increasing expression of ZO-1mRNA may play an important role.4. Repair of gastric mucosa: Immunohistochemical result shows thatthe expression of EGFR in gastric musoca significantly decreased in DRSgroup compared to control group（P<0.05）.However, exposure of DRS ratsto different doses of Huashiye Liquid resulted in increase in the expressionin EGFR in the gastric mucosa（P<0.05）.As a result, HSL could have theprotective effect on DRS-induced gastric mucosal injury via increasingexpression of EGFR in gastric mucosa.ConclusionHSL could have the ovbiously therapeutical effect on DRS-induceddysfunction of the gastrointestinal digestion and absorption,damage ofgastrointestinal barrier and metabolic disorder of liquid,regulating secretionof gut hormone and expression of some functional proteins may play animportant role.