| Background: Diabetic Nephropathy (DN) is one of the most serious complications ofdiabetes, but also the important reason, end-stage renal failure. Pathologicalchanges were glomerular hypertrophy, glomerular appear high pressure and highperfusion, high filtration, extracel the extracellular matrix (ECM), lular matrix-accumulation, basement membrane thickening, causing diffuse or tuberousglomerular sclerosis. Pathogenesis complex, many vascular active substances andcell factors involved in occur, so its pathogenesis and treatment has become one ofthe present research hotspot that need further discussion, DN.Objective: Urotensin Ⅱ(UⅡ) is the strongest peptide known vascular active peptide,its shrinking vascular efficiency can reach the endothelin-110times its specificreceptors14(14) of GPR can cause biological effects, such as vasomotion bloodvessel, promote cell proliferation and change hemodynamics etc. Research showsthat closely related with atherosclerosis, this experiment in UⅡ, GPR14discusseddiabetic nephropathy rat atherosclerosis formation action and pathogenesis. For themost commonly used medicine astragalus tonifying qi drugs, modern research thinkone of astragalus on renal has certain protective effect, but has yet to see astragalusDN rats to reports of U Ⅱ express influence. This experiment by using astragalustreatment, research of diabetic nephropathy rats UⅡ，GPR14level to explore forchange, astragalus diabetic nephropathy rat control effects and its possiblemechanism for clinical diabetic nephropathy, provide the basis for prevention andcure. Methods:1. type2diabetes nephrosis atherosclerosis form animal model of themodel group give high sugar and fat feed six weeks after intraperitoneal injectionthiourea carrizo bacteria element (chain STZ)40mg/kg.2. Group:20will be theonly model success SD rats randomized control group (DN10diabetic nephropathyonly), daily physiological saline irrigation treatment group (stomach, astragalusHZ10only), daily huangqi injection stomach5ml/kg irrigation for eight weeks.Both groups hello to fatty, sugary feed. Another set of normal control group (NC10only), daily physiological saline stomach, hello to fill based feed.14weekend,death rats under experiment.3. Determination of glucose, cholesterol, triglyceride,high-density lipoprotein cholesterol (hdl-c), low density lipoprotein, serumcreatinine, blood urea nitrogen and urinary albumin quantitative (24h).4. HEdyed light microscopy aorta, kidney pathological changes.5. immunohistochemicaltechniques UⅡ, GPR14detection protein expression, RT-PCR technologydetection UⅡ mRNA expressions.Results:1. normal control group (NC), compared to the control group (diabeticnephropathy DN group) rats in different degree, in low spirits, ingestion reduceslow, urine output increased activity, weight loss, lack luster, astragalus bodyhair treatment group (HZ) get a different degree of the cases above improvement.2. Compared with NC group, each model group kidney hypertrophy index,random blood sugar and cholesterol, triglyceride and low density lipoprotein,serum creatinine, blood urea nitrogen and urinary albumin quantitative (24h)increased significantly, high-density lipoprotein (HDL) reduced significantly, anddifference was statistically significant (P <0.01). The above indexes HZ groupgroup compared with the DN decreased obviously, there is a statistically significantdifference (P <0.05).3. Test results: DN group BNH aortic visible foam cells andfatty streaks, smooth muscle cells deformation. DN HZ group of light. More NCgroup of arterial wall endothelial cells, arranging rules the light. DN group ofkidney can be obviously glomerular hypertrophy, fibrosis and structure collapses,mesangium matrix increased, renal tubular apparent multifocal empty bubble kind change. DN HZ group of light. More NC group of glomerular, renal tubularstructure normal.4. The aorta and kidney tissue protein expression UⅡ, GPR14group compared with NC changes, UⅡ DN group, aortic protein expression increased(P <0.01). DN group compared with UⅡ HZ group protein expression reduce (P <0.05). Compared with NC group, DN group kidney tissue protein expression UⅡ,GPR14increased (P <0.01). DN group compared with UⅡ, GPR14HZ groupprotein expression reduce (P <0.05).5. Kidney tissue UⅡ mRNA expressionchanges: compared with NC group, each model group U Ⅱ mRNA express increasedsignificantly (P <0.01). UⅡ mRNA expression HZ group more DN group (P <0.05)reduce.Conclusions:1.U Ⅱ and its receptor GPR14high expression suggesting that itsatherosclerosis in diabetic nephropathy formed may play an important role in theprocess. Astragalus can alleviate kidney pathological damage, its mechanism isprobably has UⅡ and GPR14by inhibiting differentially expressed the capillarypathological changes of renal protection.2. Diabetic nephropathy astragalus canimprove the general state of rats, have a certain regulate blood sugar and blood fatmetabolism, reduce urine protein, restrain renal deteriorating effect. |
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