| Objective:Acne vulgaris is a chronic imflammatory disorder of the pilosebaceous unit that occurs most commonly during adolescence,characterised by areas of skin with seborrheal,comedones(blackheads and whiteheads),papules, pustules, nodules and possible scarring. Acne affects mostly skin with the densest population of sebaceous follicles,these area include the face,the upper part of the chest and the back.Aside from scarring,acne negatively affects psychological health and psychosocial functioning,and even quality of life.Using the lateral abdominal sebaceous glands of a golden hamster as the experimental model,traditional Chinese medicine theory as a guide, by measuring the size and observing the microscopic structure of the sebaceous glands,the objective of the experiment is to expound the formula rationality of the traditional Chinese medicine(TCM) compound "Cuo Chuang Ling"pellet, and to find out effective drugs in the compound group,in order to further the research of its hormone influencing and mechanism in acne treating.Methods:62male golden hamsters,weighing120-150g,giving normal breeding, randomly divided into six groups after a week:non-treatment control group has12golden hamsters,each of the whole prescriptions group, disassembled prescriptions group1,2, and3, positive control group has10golden hamsters.At the beginning, the bilateral abdominal feather of the golden hamsters were completely cut with an electric shaver fully to expose the bilateral sebaceous glands. The maximum diameter (DT) and maximum longitudinal diameter (DL)of bilateral plaques were measured with caliper under the strong light. The each group of hamsters was fed the drug which was10times of human dosage.Animals of the whole prescriptions group were fed with11g/kg crude drug. Disassembled prescriptions group1,2, and3were given6g/kg,2g/kg,3g/kg crude drug separately.And the positive control group were10mg/kg dosage. Non-treatment control group was given an amount (1ml/100g) normal saline. Each group of golden hamsters wes fed once daily for4weeks. At the end of the experiment, the bilateral back hair of the golden hamsters were shaved again with an electric shaver, fully revealing the bilateral sebaceous glands.DT and DL of bilateral sebaceous glands were measured with caliper under the strong light.The tissue on the sebaceous glands approximately1.5cm*1.5cm were cut off after the hamsters were executed and were put in small bottles filled with10%formaldehyde liquid. Then the sebaceous glands were paraffined, sectioned, HE dyed,and the microstructual changes was being strutinized under a light-microscope. After the golden hamsters were dissected,their testicles were weighed and the testicular coefficient was calculated.Results1. The size of sebaceous glands:The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group is smaller compared with the sebaceous glands before treating,which has a very significant difference or significant difference (p<0.01or0.05)than before.However, the non-treatment control group has no significant difference (p>0.05). The size of the left sebaceous glands of whole prescriptions group, disassembled prescriptions group1,2and positive control group is much smaller compared with the non-treatment control group,which has a very significant difference (p<0.01),and disassembled prescriptions group3with a significant difference (p<0.05). The size of the right sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2become much smaller compared with the non-treatment control group which has a very significant difference (p<0.01),while disassembled prescriptions group3and positive control group with significant differences (p<0.05). The size of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference compared with the positive control group (p>0.05). The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).2. Micro structural analysis of the sebaceous glands:The microstructure of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group loosen,becomes thinner, and the gland leaves are much smaller compared with the Blank group.Of them, whole prescriptions group, disassembled prescriptions2are obvious. Comparison of the thickness of the sebaceous glands:Thickness of the sebaceous glands of whole prescriptions group is much thinner than the non-treatment control group group.which has a very significant difference (p<0.01). Thickness of the sebaceous glands of disassembled prescriptions group2is thiner than the non-treatment control group which has a significant difference (P<0.05). Thickness of the sebaceous glands of disassembled prescriptions group1,3and the positive control group has no significant differences(P>0.05) compared with the non-treatment control group.Thickness of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant differences(p>0.05) compared with the positive control group.Thickness of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).Comparison of the loose degree of the sebaceous glands:The loose degree of the sebaceous glands of whole prescriptions group and disassembled prescriptions group1,2and positive control group is much looser compared with the non-treatment control group,which has a very significant difference(p<0.01), while disassembled prescriptions group3with a significant difference (P<0.05).The loose degree of the sebaceous glands of the whole prescriptions group, disassembled prescriptions group1,2and3has no significant difference (p>0.05) compared with the positive control group. The loose degree of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups has no significant difference compared with each other(p>0.05).3. Testicular coefficient:The testicular coefficient of whole prescriptions group and its disassembled prescriptions groups, positive control group has no significant difference compared with the non-treatment control group (p>0.05).Conclusion1. The size of the sebaceous glands of the whole prescriptions group and its disassembled prescriptions groups,positive (spironolactone) control group becomes smaller.2. The microstructure of sebaceous glands of whole prescriptions group,disassembled prescriptions group2becomes atrophy, thinner, and the gland leaves loosen. The gland leaves of disassembled prescriptions group1,3and the positive control group loosen, while the gland leaves not become thinner obviously.3. The whole prescriptions group of "Cuochuang ling" has equivalent effect with spironolactone in inhibiting hyperplasia of the sebaceous glands. The whole prescriptions group is more effective than its disassembled prescriptions groups. And the effect of inhibiting the hyperplasia of the sebaceous glands of the disassembled ones, disassembled prescriptions2group is best.next is the disassembled prescriptions group1,and the disassembled prescriptions group3is the last.4. None of the whole prescriptions group, disassembled prescriptions group1,2, and3, and positive control group has side effects of inducing atrophy to the testicular of hamsters.This shows proliferation and secrete function of the sebaceous glands can be inhibited,the sebaceous glands hyperplasia are influenced, and the golden hamsrer sebaceous glands are narrowed effectively by "Cuo Chuang Ling" through adjusting the sex hormone levels.Of the prescription,Danshen,processed Dahuang may play the main effct, followed by Pugongying,Baihuasheshecao and Gegen. |
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