A Study On The Function Of C/EBPs During The Period Of Development Of Sebaceous Gland

Sebaceous gland is an important skin appendage.Sebocytes growth and differentiation were controlled by endocrine system including androgen and many other facters,especially PPARs and C/EBPs play a key role in this progress.Sebocytes differentiation is responsed to specific ligand activators of peroxisome proliferator-activated receptors(PPARs) and involved with CCAAT/enhancer binding protein(C/EBPs).The research articles on PPARs in sebaceous gland were increased in recent years, but are still little about C/EBPs.In this study the expression of C/EBPs protein in the hair follicle .epidermis and sebaceous gland were observe ,so as to elucidate the relationship about C/EBPs and the differentiation of sebocytes.Methods: Immunhischemistry were used to detect the expression of C/EBPs protein in embryo,bornate and adult hair follicle .epidermis and sebaceous gland. And the transcription of the C/EBPs genes in sebaceous gland was analyzed with RT-PCR method.The sebocytes of human were cultured and observed with immunocytochemistry for C/EBPs.The main results of the study are as follows:The lowest expression of C/EBP α mRNA in the sebaceous gland was found in embryonic period, and increased gradually during the developmental stage .The expressions of C/EBP β mRNA in the sebaceous gland showed the different expression pattern, it maintains low level in all of developmental stages. C/EBPa and β protein expressed in the nucleus of sebocytes in embryonic period but in the basal cell layer of sebaceous gland in maturation phase.C/EBP α and C/EBP β expressed differently in the outer root sheath,follicular papilla, hair matrix in embryonic period.Its positive signals were chiefly distributed in the nucleuses of this cells.They expressed in the basal cell layer of outer root sheath in the new born and maturation phase ,the proteins expressed in nucleuses decreased with the differentiation ofouter root sheat cells,they enhanced oppositely in the cytoplasm.Ngetive stain in the follicular papilla.The expression of C/EBP a andC/EBP ￡ were mainly located in the nucleus of differentiatied cell layers in human embryonic epidermis,such as stratum spinosum, stratum granulosum and cuticula.Specific staining with C/EBPs located in the nucleuses in cultured sebocytes.Based on all the results ,Conclusion: They are expressions of C/EBP a and C/EBP 3 protein in human pilo-sebaceous unit at different stage.The expression level is closely correlated with the differentiation degree.This indicates that C/EBPs play important roles in the development of human pilo-sebaceous unit.