Effect Of The Tongguan Soup On The Changes Of VCAM-1,BAX Of The Rats With Tubal Inflammatory Obstruction

Objective:To observe the pipe side of salpingitis obstructive infertility rat oviduct epithelial cells of VCAM-1, BAX expression intensity, to explore its mechanism, to provide the basis for the clinical treatment.Methods:This experiment selected health clean8-12weeks healthy nonpregnant female Wistar rats180, placed in random permutation table method,30into normal group, model group150only. Molding using mixed bacteria inoculation, vaccination of bacterial suspension0.1ml, copy to be inflammatory obstruction of fallopian tube model. Molding20days later, the remaining125rats (died25) was arranged by table into groups:model group, Xuefu Zhuyu group, pipe, high, low dose group. Under the same condition for feeding, mould began twentieth days after intragastric administration. The normal group model group of conventional breeding, administrated with equal distilled water, Xuefu Zhuyu slice group gavage per ml of solution containing Xuefuzhuyu0.03g water solvent. Tube side, in low, high dose group were administrated through pipe, high concentration, low in water decoction. Each group was further divided into2time groups, were administered for15days,30days at two different time points for processing. And then observed by naked eyes, HE staining, in situ hybridization observation of oviduct epithelial cells in VCAM-1, BAX content change.Results:1After the mode14time points, respectively, after the model5,10,15,20days to observe the mold rat fallopian tubes, in order to judge whether the successful model.2By gavage for20days to observe the rat oviduct, found drug intervention, the rat oviduct is a significant improvement, Xuefu Zhuyu group and tube side group is better than the model group.3Treatment time, treatment for30days is better than15days, through the statistical analysis of oviduct epithelial cells in VCAM-1, BAX content, can be found through the tube side group is better than that of Xuefu Zhuyu group. In4, After15days of treatment,30days can be found in different concentration effects of different side of the tube, wherein the tube side effect of high dose group was better than the middle dosage group, middle dose group through tube is superior to that of low dose group.Conclusion:①mixed pathogen closer to clinical. The rats are more sensitive to pathogenic bacteria, survival ability. Through detection of epithelial cells in VCAM-1, BAX content, can find its change and salpingitis Obstrue Infetility occurrence, development has certain relevance. Pipe of fallopian tubal inflammatory obstructive infertility rats have a certain therapeutic effect, through the statistical analysis of the VCAM-1, BAX content that, through the tube side effect of high dose group was better than the middle dosage group, middle dose group through tube is superior to that of low dose group.30days of treatment effect is better than15days.