Effect Of The Tongguan Soup On The Changes Of IL-1,TNF-α Of The Rats With Tubal Inflammatory Obstruction

Objective:To probe the functional mechanism of the Tongguan soup and to instruct the cLinical treatment by studying the effect on the changes of the tubal morphology and cell factors(IL-1,TNF-α)of the rats with tubal inflammatory obstruction.Methods:According to random arrangement table method,30 Wistar female rats were allocated to normal group.Then the tubal inflammatory obstruction models were established by injecting the mixed bacteria.The left 150 Wistar female rats were randomly divided into 5 groups after being feeded for 20 days (except the 25 rats died after operation):model group (24 rats) controlled group (25 rats),high-dosage group (24 rats),medium-dosage group and low-dosage group with 24 rats. In the same conditions, the rats of the high-dosage group,medium-dosage group and low-dosage one were poured with various doses of Tongguan soup into stomach,the controlled group were treated with Xuefuzhuyu slice,the normal group and model group were both normal raise. Each group was divided into two time groups which were treated for 15 days and 30 days respectively before sacrifice.The pathologic identification of the tubal tissue were done by HE staining and the cytokines(IL-1,TNF-α) related to inflammation were determined in rats serum using ELISA kits.Results:1. The tubal inflammatory obstruction model was established by injecting the mixed bacteria.To confirm the model,the Pathologic identification of the tubal tissue were done by HE staining respectively after being model for 5,10,15,20days. The models were successfully established,which was confirmed by the results of HE staining and macroscopic observation.2. Compared with the model group,the controlled group and the groups by Tongguan soup can improve the tubal morphology to varying degrees.3. Compared with the model group,the controlled group and the groups by Tongguan soup can reduce the content of IL-1 and TNF-α,meanwhile increase pregnancy rates;Furthermore,the effects in the groups by Tongguan soup were superior to that in the group by Xuefuzhuyu slice, the difference between them was significant (P<0.01);4.Each groups were compared after being treated for 15d and 30d respectively:①There is no difference on the content of IL-1 between the normal group and the model group(P>0.05);There is difference on the group(P<0.01).②There is no difference on the content of TNF-αin every group (P>0.05)except the groups by Tongguan soup which have difference (P<0.01).ConcLusion:1.The tubal inflammatory obstruction model can be established successfully by injecting the mixed bacteria.2.The changes of the cytokines (IL-1,TNF-α) are reLated to the development of the infertility with Chronic oviduct inflammatory obstructive.3.The Tongguan soup can enhance the ability of disease-resistant and self-healing by regulating cytokines and improving the tubal structure and function of the rats with tubaL inflammatory obstruction,and therefore has good effects on treating the infertility with tubal inflammatory obstruction.4.The effect on the IL-1,TNF-αafter being treated by Tongguan soup for 30d is better than that for 15d.