Associations Analyses Between Single Nucleotide Polymorphisms From GWAS With Blood Pressure And Hypertension In Ethnic Study Samples

Objective: To study the association between Single Nucleotide Polymorphisms(SNPs) from genome-wide association study with blood pressure and hypertension amongUygur, Kazakh adults and Han children in Xinjiang.Method: Cross-sectional study of random population and case-control design wereapplied in our research.332cases and271controls were selected from3640Uygurs.332hypertension patients were selected from1200Kazakhs and451Han children wererecruited. Improved Miller salting-out method was used to extract DNA. Matrix-assistedlaser desorption/ionization Time-of-flight Mass Spectrometry was used to performphenotypic analysis. Hardy-Weinberg equilibrium was assessed by Chi-square test.Association of the polymorphisms with hypertension in Uygurs was tested using logisticregression. Generalized linear model was used to assess the association of thepolymorphisms with blood pressure in Kazakhs and Han children. Meta analysis wasperformed to combine the results and β was defined as effect size.Results:(1) In Uygur group, two SNPs rs11105378and rs880315were not inHardy-Weinberg equilibrium (P=0.047and P=0.006); in the Kazakh group, rs11646213was deviated from Hardy-Weinberg equilibrium (P=0.037); and in Han childrenrs381815was not in Hardy-Weinberg equilibrium (P <0.001).(2) After associationanalysis of the polymorphisms with hypertension and blood pressure in ethnic studysamples, rs11191548, rs12413409and rs995322were associated with the risk ofhypertension in Uygurs (P <0.05), but after Bonferroni correction and multiple logisticregression adjusted for gender, age, BMI, the association no longer significant. In Kazakhgroup, the association of rs1530440with blood pressure remained similar after adjustmentfor gender, age, BMI. No significant association of all the SNPs with blood pressure wasfound in Han children.(3) Meta analysis of the three groups showed that rs995322wasassociated with diastolic pressure (DBP). Conclusion: Polymorphism of rs1530440in C10orf107gene was associated with bloodpressure in Kazakhs in Xinjiang, The present of T allele was significantly associated withelevated blood pressure in Kazakhs. Polymorphism of rs995322in CSMD1gene wasassociated with DBP, and the present of A allele elevates the DBP level of Uygur, Kazakhadults and Han children in Xinjiang.