Discuss The Application Of DNA Analysis Technology In Cervical Lesions Of The Xinjiang Uygur Women

Objective: To analyse Xinjiang Uygur women’s cervical exfoliative cells samples byflow cytometry (FCM) DNA analysis, combined with its result of thin layer of liquid andcytology test (TCT) and the human papilloma virus (HPV) examination, in order todiscuss the application value of flow cytometry DNA analysis in diagnosis and screeningfor cervical lesions. Methods: Collected the TCT remnants specimens of the Uygurwomen which initial visit the gynecological from April to August,2011, in CytopathologyRoom of Tumor Hospital Affiliated Xinjiang Medical University; At the same timecollected specimens of HPV test, then HPV type testing and half quantitative test.Altogether collected162cases. Results:1. A positive correlation between HPV-DNA andDI/SPF of cervical epithelial cells (rsis0.807and0.827, P<0.05), along with theincreasing of cervical lesions, virus load alao increased with the epithelial cells DNAploidy increasing.2. Compared HPV negative infection, It is17.273times and32.575times than appearing DNA aneuploid in single high-risk type HPV infection and mixedhigh-risk type HPV infection.3. Compared TCT normal group, It is respectively8.143times and180.500times than appearing DNA aneuploid in moderate lesions group andhigh lesions group.4. Follow-up the4suspicious cases of DNA analysis, after6months,then found that two cases can be diagnosed as cytology lesions by TCT review.Conclusion:1. The result of Flow cytometry DNA analyse has a good consistency withTCT result and HPV test result;2. It is help to develop better screening cervical lesions inXinjiang Uygur women through joining flow cytometry DNA analysis into the presentprogram screening of cervical lesions.