Preliminary Investigation On The Detection Of Human Papilloma Virus And The Diagnosis Of Cervical Lesions Using Surface Plasmon Resonance

Objective:To investigate the value of HPV diagnosis in cervical lesion with a new type of human papilloma virus detection techniques--surface plasmon resonance technology(SPR).Methods:we screened cases with TCT first,from November 2008 to June 2009 in the Second Xiangya Hospital gynecological clinic.287 patients who are at least ASCUS TCT diagnosis were random choiced to detecte HPV with SPR, examed with colposcopy and biopsy. At the same time we detected HPV in 157 patients with Flow-through hybridization and gene chip technology (HybriMax), The data were analyzed with SPSS 13.0 software package for Chi-square test analysis and Spearman rank correlation analysis.Results:①During the 287 cases we found out HPV in 117 cases by SPR, of 157 cases consistent with HybriMax, SPR sensitivity, speciality, positive and negative predictive value are 100%,80%,93.6%,100% respectively. HPV positive rate of detection with SPR is 94.90% and showed no significant difference with HybriMax(p> 0.05).②TCT single test, TCT+SPR HPV-positive patients with joint detection, cervical intraepithelial neoplasia and cervical carcinoma were 45.30%,61.67%, with significantly different between (P<0.05).③With CINI,Ⅱ,Ⅲand cervical cancer patients with HPV detection rates were 67.50%,92.31%,96.30%,96.77%, and the rate of the TCT results of ASCUS,LISL,HISL and Cervial cancer cases were pathological levels were 46.24%,88.57%,91.30%,100%. By Spearman correlation analysis, was positive correlation r=0.0487, p=0.000,α= 0.05.Conclusion:①The Surface plasmon resonance technology is an effective way in the detection of cervical lesions,it is should widely promoted in clinical practice.②Using SPR detection of HPV virus and associated with Thin prep cytology test and colposcopy can reduce the rate of missed diagnosis of cervical lesions and improve its accuracy.