Change Of CTnI、CK-MB And MicroRNA-1Level In Perioperative Blood From Patients With Cardiopulmonary Bypass Open Heart Surgery

Objective：To investigate the effect of serum cTnI, CK-MB, cardio-specificmicroRNA-1(miR-1) on the diagnosis of ischemia/perfusion(I/P) injury incardiopulmonary bypass open heart surgery patients. Methods：Pre-operative,Intra-operative(protamine reverse heparin) and6h post-operative plasmasamples were collected from20cardiopulmonary bypass open heart surgerypatients. Serum miR-1levels were measured with TaqMan real-timeQuantitative Polymerase Chain Reaction Detecting system (qRT-PCR).BloodcTnI and CK-MB levels were measured at the same time. Results：Serum cTnIor CK-MB levels in intra-and6h post-operative blood were significantly higherthan that in pre-operative blood(P<0.05), and serum cTnI or CK-MB levels inintra-operative blood were still significantly higher than that6h post-operativeblood(P<0.05). Compared with the pre-operative serum miR-1levels, intra-andpost-operative serum miR-1levels were obviously increased(8-16fold)（P<0.05）,but serum miR-1levels between intra-and post-operative bloodhave no statistical difference(P>0.05). Conclusion：Serum cTnI or CK-MB is avaluable marker for immediate myocardial damage in cardiopulmonary bypassopen heart operation. Serum miR-1is a stable biomarker for cumulativemyocardial damage during myocardial ischemia/perfusion in cardiopulmonarybypass open heart operations.