| Objective:Apply micro channel array flow analyzer to observe transiting time and erythrocyte rheology changing characteristics of elderly hypertension patients with different Traditional Chinese Medicine syndrome. Analyse elderly hypertension patients’relationship between micro channel array flow transiting times and every indexs.Methods:Testing109patients micro channel array flow transiting time, erythrocyte rheology index and laboratory relevant indexs, and according to Traditional Chinese Medicine syndrome differentiation, divided109patients into phlegm-stasis blocking collateral group (35cases), yin deficiency with yang hyperactivity group (42cases), kidney deficiency group (32cases). And there is a elderly healthy group (21cases). Variance analysis, LSD multiple comparison analysis were used to analyse the relationship among the three groups. Relevant analysis and multiple regression analysis were used to analyse109elderly hypertension patients’relationship between micro channel array flow transiting times and every indexs.Results:1Micro channel array flow transiting time comparison:elderly hypertension group (n=109)10ul transiting time:9.88±2.15s,30ul transiting time:33.86±8.35,60ul transiting time:70.65±8.31s,100ul transiting time:136.66±31.53s; phlegm-stasis blocking collateral group (n=32)10ul transiting time:11.04±2.27s,30ul transiting time:37.49±7.70,60ul transiting time:79.70±6.71s,100ul transiting time:174.23±9.18s; yin deficiency with yang hyperactivity group (n=42) lOul transiting time:9.54±2.01s,30ul transiting time:32.31±6.14s,60ul transiting time:67.74+7.57s,100ul transiting time:113.78±4.74s; kidney deficiency group (n=32) lOul transiting time:8.95±1.56s,30ul transiting time:32.07±10.05s,60ul transiting time:61.51±5.13s,100ul transiting time:111.52±5.05s; elderly healthy group (n=21) lOul transiting time:8.34±1.76s,30ul transiting time:28.25±9.28s,60ul transiting time:61.18±5.53s,100ul transiting time:109.01±8.18s. The micro channel array flow transiting time of phlegm-stasis blocking collateral group compare with the micro channel array flow transiting times of yin deficiency with yang hyperactivity group and kidney deficiency group and elderly healthy group, the comparison of statistics has significant difference (P<0.05).2The erythrocyte rheology index comparison:when shear rate is100s-1, erythrocyte deformation index of phlegm-stasis blocking collateral group compare with yin deficiency with yang hyperactivity group and kidney deficiency group and elderly healthy group was reduced and the statistical comparison has significant difference (P<0.05); when shear rate is1500s-1, deformation index of phlegm-stasis blocking collateral group erythrocyte compare with yin deficiency with yang hyperactivity group and kidney deficiency group and elderly healthy group was reduced and the statistical comparison has significant difference (P<0.05); integrated erythrocyte deformation index of phlegm-stasis blocking collateral group compare with yin deficiency with yang hyperactivity group and kidney deficiency group and elderly healthy group was reduced and the statistical comparison has significant difference (P<0.05); erythrocyte aggregation index of phlegm-stasis blocking collateral group compare with yin deficiency with yang hyperactivity group and kidney deficiency group and elderly healthy group was increased and the statistical comparison has significant difference (P<0.05).3Erythrocyte related plasma ATPase activity comparison:plasma Na+-K+-ATPase activity of phlegm-stasis blocking collateral group and yin deficiency with yang hyperactivity group and kidney deficiency group compare with elderly healthy group was reduced, the statistical comparison has significant difference (P<0.01); among the three groups with different Traditional Chinese Medicine syndrome, the statistics comparsion has no significant difference (P>0.05). Plasma Ca2+-Mg2+-ATPase activity of phlegm-stasis blocking collateral group and yin deficiency with yang hyperactivity group and kidney deficiency group compare with elderly healthy group was reduced, the statistical comparison has significant difference (P<0.01); plasma Ca2+-Mg2+-ATPase activity of phlegm-stasis blocking collateral group compare with kidney deficiency group was reduced,the statistical comparison has a significant difference (P<0.05), but compared with yin deficiency with yang hyperactivity group the statistics has no significant difference (P>0.05).4The correlation of109elderly hypertension patients’micro channel array flow transiting times and indexs:the lOul transiting time,30ul transiting time,60ul transiting time and100ul transiting time were respectively significant negative correlated with integrated erythrocyte deformation index(r=-0.396, P=0.041; r=-0.602, P=0.006; r=-0.739P=0.000; r=-0.780, P=0.013), lOul transiting time,30ul transiting time and60ul transiting time were respectively significant negative correlated with erythrocyte deformation index when shear rate is1500s-1(r=-0.507, P=0.007; r=-0.490, P=0.033; r=-0.484, P=0.031),30ul transiting time,60ul transiting time and100ul transiting time were respectively significant negative correlate with erythrocyte deformation index when shear rate is450s-1(r=-0.524, P=0.021; r=-0.707, P=0.000; r=-0.825, P=0.006),100ul transiting time was significant positive correlated with erythrocyte aggregation index. lOul transiting time,30ul transiting time, and100ul transiting time were respectively significant negative correlated with plasma Ca2+-Mg2+-ATPase activity (r=-0.339, P=0.002; r=-0.265, P=0.038; r=-0.613, P=0.045), but micro channel array flow transiting time was unrelated with plasma Na+-K+-ATPase activity.30ul transiting time was significant positive correlated with mean corpuscular volume (r=0.350, P=0.002), and was significant positive correlated with red blood cell distribution width standard deviation (r=0.329, P=0.013), but micro channel array flow transiting time was unrelated with red blood cell count, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration and red blood cell distribution width standard deviation-coefficient of vaviation. Micro channel array flow transiting time was unrelated with total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, carotid artery intima-media thickness.60ul transiting time and100ul transiting time were significant positive correlated with waistline (r=0.438, P=0.010; r=0.480, P=0.044),60ul transiting time and100ul transiting time were significant positive correlated with body mass index (r=0.529, P=0.001; r=0.588, P=0.010). Conclusion:MC-FAN visualized blood flow image and transiting time can be used as laboratory diagnosis references of elderly hypertension, especially phlegm-stasis blocking collateral group. |
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