| Nitric oxide(NO) is a new cell-messenger molecule with strong biologic activity and has become the latest hot spot in the field of transfusion medicine. It is the gas molecules with free radical nature and combined with Hbβ93Cys to form SNO-Hb through the erythrocyte membrane. NO bound in Hb can regulate the storage of itself and also the release of it to mediated vasodilation from heme to Hbβ93Cys. The main effectiv factors of erythrocyte deformability is cell volume, cell shape, cytoplasmic viscosity and membrane mechanical properties. Although the lipid bilayer and the cytoskeleton of RBC can affect the mechanical behavior of erythrocyte membrane, the physical properties of membrane skeleton is the determined factor of the membrane viscosity and elasticity. Adverse changes of banked blood has been extensively studied and confirmed. In particular, the decline of RBC deformability leads to the dysfunction of stored blood, this result of change may jeopardize the safety of clinical blood transfusion and may be even harmful for the potential recipients. Therefore, how to improve the hemorheology of banked blood has become imperative traits matter. The interaction of NO and hemoglobin may lead a series changes from the conformation change of RBCs membrane skeleton protein to changes RBC deformability. This study is try to improve the deformability of RBCs by adding NO and to explore the effect characteristics of NO on erythrocyte Deformability.Objective: To exploring mutual relations of NO and erythrocyte deformability by studying the effective characteristics of erythrocyte Deformability with different NO concentrations added NO donor in banked blood during different times of storage. Methods : Blood samples(200mL/bag) were collected from the ten healthy men. 20mL blood from each samples were distributed to five tubes(4mL/tube). Four tubes blood samples were incubated with four different concentrations SNP separately in 8-phase (3h, 1d, 3d, 5d, 7d, 14d, 21d, 28d) of storage times.SNP concentrations were 1μM (A group), 5μM (B group) , 10μM (C group), 50μM (D group) separately. The last tube was the control one without any treatment. At the same time, 1mL blood sample drawn from blood bag was used to detect the NO concentration at each time, The multiparameter analysis of hemorheology(high-shear viscosity, low shear viscosity, Casson viscosity, hematocrit, erythrocyte deformability index, aggregation index, stiffness index) was been tested for each group.Results: Hematocrit of control blood samples had no significant changes in storage period. Erythrocyte deformability index, aggregation index and stiffness index were gradually increased with time prolonged during storage of banked blood. Whole blood relative viscosity in high-shear was significantly increased at 1d, and then maintain the trend of increasing. Whole blood relative viscosity in low-shear was no change from 3h to 5d, and significantly increased at 7d, and then maintain the trend of increasing. Casson viscosity was significantly increased at 1d, and then maintained the trend of increasing also. When the SNP concentration was 5μM or 10μM,erythrocyte deformability index in 7d or more than 7d storage of banked blood cells was significantly degraded. Higher than the best range of NO concentrations, the erythrocyte deformability index was significantly increased. When the SNP concentration was 5μM, erythrocyte rigidity index was changed significantly compare to the control group from 3d to 7d. When the SNP concentration was 10μM, erythrocyte rigidity index was changed significantly compare to the control group from 14d to 28d. When the SNP concentration was 50μM, erythrocyte rigidity index was changed significantly compare to the control group after 5d. The erythrocyte aggregation index with a control value did not significantly change when blood was incubated with different SNP concentrations.Conclusion: Hemorheology traits of banked blood was changed significantly during storage of banked blood. Erythrocyte deformability index and aggregation index were decreased significantly. NO can significantly impact on the erythrocyte deformability with dual-phase.when NO is an optimum concentration, erythrocyte deformability is improved significantly. Higher than the best concentration, erythrocyte deformability is damaged . The storage quality of banked blood can be improved significantly by adding the best concentration of NO.
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