Study On The Relationship Between SFRP1and Chronic Periodontitis

Object:This study is made to detect the concentration of SFRP1in gingival crevicular fluid (GCF) which is token from both patients those with chronic periodontitis (CP) and healthy, expression of SFRP1in the gingival tissue. Discover the relationship between SFRP1and the occurrence and development of chronic periodontitis.Methods:1. Twenty-eight patients with chronic periodontitis were selected as CP group and ten healthy volunteers were enrolled into the study as control group. GCF were collected, and three periodontal clinical parameters were selected and recorded, including the bleeding index (BI), periodontal probing depth (PD) and clinical attachment loss (CAL). The concentration of SFRP1in GCF samples was detected by Enzyme-linked immunosorbent assay (ELISA).2. Twenty-two patients with chronic periodontitis were selected as CP group and ten healthy volunteers were enrolled into the study as control group. Gingival tissues biopsies were token and immunohistochemistry analysis for SFRP1was used. Data were analyzed by the SPSS19.0software.Results:1. The weight of GCF of chronic periodontitis group (1.21±0.61mg) was significant higher than the normal control group (0.58±0.29mg)(p<0.05).2. The concentration of SFRP1in GCF of CP group (281.07±33.37ng/L) was significantly higher than the control group (245.30±35.67ng/L)(p<0.05).3. There was a significant positive correlation between the concentration of SFRP1in GCF and CAL 4. The concentration of SFRP1in GCF of the mild chronic periodontitis group was250.42±19.48ng/L, while in the moderate chronic periodontitis group:285.70±21.29ng/L, and in the severe chronic periodontitis group:306.57±32.50ng/L. The mild group was significantly lower than moderate and severe group (p<0.05); The last two groups (moderate and severe) was no significant (p>0.05).5. In the epithelial tissue cells, the SFRP1were positively detected in both the control and CP groups. While in the connective tissue, the SFRP1was expressed in the CP group, which is noticed especially in the endothelial cells, fibroblasts and inflammatory cells. In contrast to the control group were its weakly expressed.6. The SFRP1-positively stained in all the gingival tissue specimens of CP group, however it’s stained positively in about80%of the control group. The scores of SFRP1in CP group (4.50±0.91) were significantly higher than the control group (2.80±1.14), p<0.001. But it was no significant among mild, moderate and severe CP group (p>0.05).7. The LI of inflammatory cells (23.29±8.33) and fibroblasts (6.46±3.19) of CP group were significantly higher than those of the control group respectively (5.82±3.18and1.89±1.37)(p<0.05).Conclusion:1. The concentration of SFRP1in GCF of CP group was significantly higher than that of the control group.2. The level of SFRP1in GCF was increase with the severity of periodontal destruction.3. The expression of SFRP1in CP group was significantly more than the control group. 4. SFRP1probability played a promoting role in the development of chronic periodontitis.