Detection And Clinical Benefits Of Interleukin-4 And Interleukin-17 In Gingival Crevicular Fluid In The Patients With Periodontitis

Objective: The cytokine has an effect of immunoregulation and immediate induction in the destruction pathology of periodontitis. In this experiment, the content of Interleukin-4 and Interleukin-17 in healthy periodontal sites and in periodontitis sites before and after periodontal treatment were measured by enzyme-linked immunosorbent assay (ELISA). The relationship was discussed among Interleukin-4 and Interleuki-17 in GCF and clinical parameters. The purpose of this study is to research the relationship among Interleukin-4 and Interleukin-17 in GCF and periodontitis ;to explore that Interleukin-4 and Interleukin-17 are regarded as beneficial indexes to reflect the periodontal status or not; and to explore the effect of Interleukin-4 and Interleukin-17 in periodontitis pathology and the illness progress.Methods: 15 Subjects who had periodontitis in this study were selected as the case group, 10 healthy volunteers who had no periodontal disease were selected as the normal group .A molar tooth was selected as the study object from all the subjects. The case group was devided into pretherapy and post- treatment through periodontal initial therapy. To observe and record the clinical parameters: GI, PD ,AL of every selected tooth. GCF was collected by paperstrip , ELISA was used to detect the levels IL-4 and IL-17 in GCF ,and an correlation check was done between the clinical parameters and the concentration of IL-4 and IL-17.Results: (1) The concentration of IL-4 in GCF in the inflammatory group was significant lower than the normal group(P<0.05). No significant deference was found between Pretherapy and post-treatment through periodontal initial therapy(P>0.05), while significant deference was found between post-treatment and healthy control(P<0.05). (2) The concentration of IL-17 in GCF was significant higher than the normal group(p<0.05), significant deference was found between Pretherapy and post-treatment through periodontal initial therapy, and no significant deference was found between post-treatment and heathy control. (3) The clinical parameters showed significant deference between the inflammatory group and the normal group or pretherapy and post-treatment. (4) Negative correlation was found between PD and the level of IL-4 in GCF, The correlation coefficient was -0.671 ,(p=0.006); while the levels of IL-4 in GCF was not significantly associated with the clinical parameters GI and AL, the correlation coefficient were -0.182, (p=0.517); -0.016, (p=0.954); Strong positive relationships were found between the level of IL-17 in GCF and the clinical parameters ( GI PD AL), The correlation coefficient were 0.545, (p=0.036); 0.537, (p=0.039); 0.767, (p=0.001).Conclusion: There existed IL-4 and IL-17 in GCF of periodontitis patients. The concentration of IL-4 in periodontitis sites was significantly lower than in healthy sites,while the concentration of EL-17 in periodontitis site was significantly higher than in healthy sites. Through periodontal initial therapy, the level of IL-4 did not increased significantly, while the level of IL-17 decreased apparently .The decrease of IL-4 levels and increase of IL-17 levels can reflect the degree of the inflammatory periodontal disease, So the two factors played a role in the cause and development of periodontitis .This experiment proved the theory that deficiency of IL-4 can result in periodontitis. We can regard IL-4 as a sensitive diagnosis index of periodontitis. It was identical that the level of IL-17 in GCF with the improvement of the degree of periodontal inflammation, and it can be regarded as beneficial indexes that reflect the status of periodontitis.