Effects Of Neuromuscular Facilitation Technique On Bone Mineral Density Of The Hemiplegia Limb And Expression Of Serum Leptin Levels Following Cerebral Infarction In Rats

[Backgroud and Objective]Stroke patients who is abed in long-term and muscle dynami c anomalies can cause different degree of osteoporosis, particularly the hemiplegia limb, and they are apt to fall, which induce more higher rate of fracture incidence and lower quality of life. The treatment of osteoporosis after stroke will become new direction in modern rehabilitation. In the present study, we investigate the effects of neuromuscular facilitation technique on bone mineral density of the hemiplegia limb and expression of serum leptin levels following cerebral infarction.,which may provide possible evidence in prevention and treatment of stroke secondary osteoporosis with neuromuscular facilitation technique.[Methods]Acute cerebral infarction was modeled in aged female Wistar rats using the permanent middle cerebral artery occlusion(PMCAO)technique。 The rats were randomly divided into sham operation, control, conventional treatment, conventional treatment combined with neuromuscular facilitation technique groups (each group had10rats).The condition of osteoporosis was examined by measuring bone mineral density (BMD) of the proximal paralyzed limb, serum total alkaline phosphatase (ALP), bone alkaline phosphatase (BALP), osteocalcin (OC), interleukin-6(IL-6) afrer treatment for0.5,1,2, and3months. In addition, the expression of Leptin ((LP) was determined by enzyme-linked immunosorbent assay at different time points.[Results]Compared with the control groups, the expression of serum BGP、 BALPandALP in the conventional treatment combined with neuromuscular facilitation technique groups was significantly higher from treatment for1month. Further studies demonstrated that the expressions of serum BALP、ALP and bone mineral density were remarkably increased in the conventional treatment combined with neuromuscular facilitation technique and conventional treatment group from treatment for2month in contrast; it was higher in the conventional treatment combined with neuromuscular facilitation technique group than that in the conventional treatment group from treatment for3month. As compared with the control group, the expression of serum IL-6was decreased in the conventional treatment combined with neuromuscular facilitation technique group from treatment for1month, and that was decreased in the conventional treatment group from treatment for2month, there was no significant difference between the sham operation and the conventional treatment combined with neuromuscular facilitation technique groups. As compared with the control group, the expression of serum IL-6in the sham operation and two treatment groups from treatment for3month showed no significant difference. The expression of serum LP was significantly higher in two treatment groups than in the control group from treatment for1month, and it was significantly higher in the conventional treatment combined with neuromuscular facilitation technique group than in the conventional treatment group from treatment for2month; there was no significant difference between the sham operation and the conventional treatment combined with neuromuscular facilitation technique groups from treatment for3month。[Conclusion]1. Neuromuscular facilitation technique can improve hemiplegia limb bone mineral density after cerebral infarction2. Neuromuscular facilitation technique can increase serum BGP, BALP, ALP levels and decrease serum IL-6level3. Neuromuscular facilitation technique can promote bone mineral density and prevent osteoporosis occurrence, which may be associated with up-regulation of the expression of LP to improve bone formation and inhibit bone absorption...