The Influence Of Wenyang Tonglin About Cell Adhesion Effect Of Uropathogenic E.coli Dealed With Urethral Epithelial Cell In Vitro

Purpose: Discussed the influence of Wenyang Tonglin about cell adhesion effect ofUropathogenic E.coli dealed with urethral epithelial cell in vitro.Method: Make up water solubility micromolecule hydrone material of traditionalChinese medicine according to the rule of Wenyang Tonglin.Separated Uropa-thogenic E.coli from medical laboratory technolongy,detectioned virulence gene ofUropathogenic E.coli,used Polymerase Chain Reaction augmented the virulen-ce gene papC、Hly、Cnf1、Aer of Uropathogenic E.coli J96.Cultivated human urethralepithelial cell used Original generation of cell culture improved.Watching the influence ofWenyang Tonglin about cell adhesion effect of Uropathogenic E.coli deal with urethralepithelial cell in vitro.Result: Virulence gene NDA were Positived control between UPEC J96and standardUPEC,it proofed that papC、Hly、Cnf1、Aer were excellent virulence gene of UPEC.The cellsform accord with the form of urethral epithelial cell,which cultivated by Original generationof cell culture improved,it proofed that the method Original generation of cell cultureimproved was feasibilitide.The water solubility micromolecule hydrone material of traditionalChinese medicine according to the rule of Wenyang Tonglin can reduced Adhesion effecteffectively,witch between UPEC J96and urethral epithelial cell in vitro(P<0.05);the strengthof the drug in proportionaled to the concentration of drug（P＜0.01);the adhesion effectbetween UPEC J96and urethral epithelial cell in vitro increased prominent with time in3ominite（P＜0.05）,the adhesion effect between UPEC J96and urethral epithelial cell in vitrostill increase by degrees after30minite，but there are not significant difference between30minite（P>0.05）；the adhesion rate and the adhesion index of control group UPEC were all0at any time.Conclusion: Water solubility micromolecule hydrone material of traditional Chinesemedicine according to the rule of Wenyang Tonglin can reduced adhesion effect effectively，Which between UPEC J96and urethral epithelial cell in vitro,this was one of mechanismtreated urinary tract infection.