Antitumor Effect Study Of The Chinese Preparation Xian Ci Dan

Background and objectThe antitumor preparation Xian Ci Dan (XCD for short), which is according to the basic theory of Traditional Chinese Medicine and efficacious prescriptions in clinic, is designed by some Chinese Medicine hospital in Guangzhou. It showed significant efficacy towards lung cancer, gastric cancer, liver cancer, nasopharyngeal carcinoma and so on, given administrated alone or as assistant to chemotherapy. Furthermore, it also showed great efficacy for cancer patient of last period, who was treated with chemotherapy with no efficacy. In this study, we took the A459cells as researching object in vitro, to investigated XCD’s antitumor effect in the aspects of its depression on the proliferation, apoptosis and cycle of cancer cells. For experiments in vivo, we studied Lewis tumor-bearing mouse model and S180tumor-bearing mouse model to investigated XCD’s antitumor mechanism, in the aspects of life quality, tumor inhibition rate, apoptosis and cycle of transplanted cancer cells, angiogenesis and so on.In a word, this study provided scientific theoretical evidence for XCD’s cancer curing effect in clinic appliance.Method and result1. Fxtraction of effective fraction of XCD.The XCD pill was smashed into powder, extracted by water, precipitated by othanol, then filtrated and condensed. Afterwards, the extraction was purified by column chromatography with AB-8macroporous resins. The80%ethanol part of elution was collected, condensed and dried to gain the XCD effective fraction powder, with an acquiring rate of0.90%.2. XCD’s effect on A549cells Different concentration of XCD effective fraction were administrated to the A549cells. Its suppression rate against the A549cells was determined with MTT method to calculate the IC50. the IC50of24h,48h and72h were136.55μg/mL、56.671μg/mL、55.322μg/mL, respectively. The methods of Annexin Ⅴ-FITC/PI were applied to determine XCD effective fraction’s effect on the apoptosis and cycle of the A549cells. It come out with the result that XCD could inhibit tumor cell’s proliferation by inducing cell apoptosis and blocking the initiating of cell cycle.3. XCD’s effect on Lewis tumor-bearing mouseIn this study, the Lewis tumor-bearing mouse model was built to observe XCD effective fraction’s antitumor effect in vitro. The methods of Annexin Ⅴ-FITC/PI were applied to determine XCD effective fraction’s effect on apoptosis and cycle of transplanted cancer cells. Expression of p53, Bcl-2in tumor tissue were determined by western-blot method, while that of CD34and VEGF were determined by immunohistochemistry. Result showed that at medium and low dose, XCD could improve the immunity of tumor-bearing mouse. Through inducing cell apoptosis and blocking cell cycle, as well as inhibiting angiogenesis, XCD effective fraction could restraining the tumor.4. XCD’s effect on S180tumor-bearing mouseThe S180tumor-bearing mouse model was built to observe XCD effective fraction’s antitumor effect in vitro. The methods of AnnexinV-FITC/PI were applied to determine XCD effective fraction’s effect on apoptosis and cycle of transplanted cancer cells. Expression of p53, Bcl-2in tumor tissue were determined by western-blot method, while that of CD34and VEGF were determined by immunohistochemistry. It come out with a result that at medium and low dose, XCD could improve the immunity of tumor-bearing mouse. By inducing cell apoptosis and blocking cell cycle, as well as inhibiting angiogenesis, XCD effective fraction could restraining the tumor.ConclusionXCD showed significant antitumor effect experimentally in vitro and in vivo. It could improve life quality and enhance adaptability of tumor-bearing mouse. One of its mechanism might he down-regulating the expression level of mutant p53, Bcl-2in tumor cells. It could also down-regulating the expression level of CD34and VHGE, reducing the density of micro blood vossels in the tumor, therefore restraining the tumor.