Screening Of Antitumor Active Fraction Extracted From Rumex Gmelini Turcz. And Experimental Studies

Rumex gmelini Turcz.is classified as Rumex(Polygonaceae).Its root and rhizome have been used for antifungal,antitumor,antitussive and expectorant, antiasthma,antivirus and antioxidation.To understand its antitumor activity and to reveal its antitumor mechanism,we carried out this experiment.In the first part of experiment,we studied in vivo the effective fraction from Rumex gmelini Turcz.on the inhibiting rate.Compared with the control group, treatment-group has tumor-inhibitory effect in vivo of the Rumex gmelini Turcz.n-butanol extract and aqueous extract to S₁₈₀ and U₁₄ mice;but the Rumex gmelini Turcz.ethyl acetate extract has no influence on the tumor-inhibitory.In the second part of experiment,we studied in vitro the Rumex gmelini Turcz.n-butanol extract and aqueous extract on the inhibiting effect on the tumor cell lines of human.The results indicated the n-butanol extract strongly inhibited the tumor growth of homo HT-29 colon cancer cells,homo HCT-116 colon cancer cells,homo Hep G-2 liver cancer cells,homo SPC-A-1 lung cancer cells,homo SGC-823 stomach cancer cells;the aqueous extract strongly inhibited the tumor growth of homo HT-29 colon cancer cells,homo HCT-116 colon cancer cells,homo Hep G-2 liver cancer cells,homo SPC-A-1 lung cancer cells;and there is dose-dependent relation in these cells treated with the n-butanol extract and the aqueous extract in vitro.In the third part of experiment,we detected the morphology change of HT-29,HCT-116,Hep G-2 and SPC-A-1 cells which were treated with different dosages of the aqueous extract by Apoptosis and Necrosis Assay Kit. Then we observed the morphology change of HT-29 and HCT-116 cells which were treated with different dosages of the aqueous extract by transmission electron microscope.The results indicated that the aqueous extract from Rumex gmelini Turcz.could induce homo tumor cells'apoptosis.The results of these studies can offer the theoretic base to clinic application of Rumex gmelini Turcz.in tumor treatment in the future.