| Background:Rectum cancer is one of the malignant tumors which seriously threaten human being. As the people’s living standards enhances and lifestyle changes, incidence and mortality rates of colorectal cancer are increased every year. Tumor growth and metastasis can not be separated from angiogenesis. Now the anti-angiogenic become a new method of treatment of cance after surgery, radiotherapy, chemotherapy and biological therapy. Many studies have confirmed that VEGF blockers inhibit tumor growth and show effective in angiogenesis, but not all tumors are sensitive to the VEGF blockers, even those who initially are sensitive will become the development of drug resistance. Therefore, we need to find a new target in angiogenesis.BFGF is one of the major factor in promoting tumor angiogenesis. BFGF plays a biological effect mainly through the cell membrane receptor with high affinity receptor (FGFR). FGFR2is one of the high affinity receptors. Malignant tissues highly express bFGF and bFGF receptor through autocrine and paracrine, which directly role on a variety of cells and then result in formating tumors through inducing cell transformation, uncontrolled proliferation and abnormal differentiation. Therefore, the targeted drugs in human malignant tumors has not only a potential capacity of anti-angiogenic, but also has direct anti-tumor effect. In the research we designe to detect FGFR2protein which express in rectum cancer tissue, paired adjacent tissues and normal tissues, and analyze the relationship with clinicopathologic parameters and preoperative CEA.We explore FGFR2in the development of colon cancer,and provide some theoretical basis in the role of molecular targeted therapy for cancer.Materials and Methods:70patients who underwent surgery of rectum carcinomal at the Affilicated Taizhou Hospital between2008.12-2011.5were induced in the study.34patients with adjacent tissues (the distance to the edge of adenocarcinoma between1.5cm and2.0cm, confirmed pathologically as normal tissue) and normal tissue (the distance to the edge of adenocarcinoma more than10cm.confirmed pathologically as normal tissue), TheHuman Tissue Bank of Taizhou Hospital provide all of samples.None of the patients had received chemotheraphy or radiation therapy before surgery.The expression of FGFR2protein was determined by immunohistochemistry.The data was analyzed by SPSS17.0.Nonparametric test and Spearman correlationwere used to analyze the difference between groups.a=0.05was considered as statistically singificant value.Results:1. FGFR2expressionThe expression of FGFR2in34cases of rectum adenocarcinoma and paired adjacent tissues, normal tissue. There was significant difference in cancer tissue compared with normal tissue or adjacent tissue (P<0.01). There was not significant difference between Adjacent tissue and normal tissue (P>0.05).The expression of FGFR2protein in rectum adenocarcinoma tissue was correlated with lymph node metastasis、clinical staging and preoperative CEA (p<0.05) without relationship with gender, age, tumor size, tumor location, Tumor type, histological grade, tumor invasion depth ang vascular thrombosis (P>0.05).The relationship between the expression of HIF-la and NDRG-1was positive by spearman correlation analysis(r=0.371, P<0.05).2. the relationship between clinical pathology parameters and preoperative CEAPreoperative CEA was correlated with lymph node metastasis and clinical stage, P values are0.0052, without relationship with other pathology parameters; The vessel invasion was correlated with lymph node metastasis and clinical staging, P values are0.0002; tumor size was correlated with tumor location, P=0.0168.Conclusion1、The expression of FGFR2in rectum adenocarcinoma was significantly higher than the paired adjacent tissue and normal tissue; The expression gradually increased with the Lymph node metastasis, increasing clinical staging, increasing, preoperative CEA The differences all have statistical significances indicating FGFR2playing an important role in rectum cancer occurrence, development and metastasis.2、FGFR2had positive correlation in rectum adenocarcinoma, indicating their participation in the occurrence, development and metastasis of the gastric adenocarcinoma.3、Preoperative CEA was significantly correlated with lymph node metastasis and clinical stage suggesting that it may be as an indicator to determine the prognosis of patients before surgery; vessel invasion was significantly correlated with lymph node metastasis, suggesting that it may be an indicator to determine the prognosis of patients. |
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