Levels And Clinical Significance Of Mip-1α、ECP In Serum From Children With Bronchiolitis

Background: Bronchiolitis is the specific respiratory diseases to infantsunder2years old. It is caused mainly by respiratory syncytial virus infection.The major clinical manifestations is cough, dyspnea and wheezing, severe casescan be complicated by respiratory failure, heart failure and it can seriouslythreaten the lives of children. Nowadays, there is no effective treatment for thisdisease, and the main treatment is symptomatic and supportive therapy. Thepathogenesis of bronchiolitis is very complex, a variety of immune cells andimmune molecules are involved in its pathogenesis. MIP-1α belongs to CCchemokine family, that is β-chemokine family. The main role of MIP-1α ischemotaxis of CD4+T cells and CD8+T cells, monocytes, eosinophils, basophils,mast cell infiltration, and it can enhance the activity of them. Activated immunecells can release a variety of inflammatory mediators and can damage epithelialcells of airway. In addition, MIP-1α can induce eosinophils to release the ECP,and it can stimulate mast cells, basophils to release histamine. ECP is a basicprotein released by activated eosinophils, and it is the sign of eosinophilactivation. ECP can induce mast cells to release histamine, and it can damageairway epithelial cells directly, leading to cell shedding, causing hyperrespo-nsiveness of airway. As for the role of MIP-1α in bronchiolitis, there are fewreports at home and abroad. However, whether the level of serum ECPincreased is still controversial, different scholars hold different attitudes. Tofurther study the role of MIP-1α and ECP in bronchiolitis, we detecte the levelsof MIP-1α and ECP in surm from children with bronchiolitis.Objective: To investigate the levels and clinical significance of MIP-1α,ECP in serum from children with bronchiolids and analyze the correlation between the levels of MIP-1α, ECP in the acute phase of bronchiolitis and themore recurrent wheezing after recover from the bronchiolitis.Methods: The object of this study is enrolled with bronchiolitis in in thedepartment of pediatrics of the the First Hospital of Jilin University fromNovember2010to December2011. Select the children without infection duringthe same period of hydrocele of testis, inguinal hernia, umbilical hernia in theDepartment of Pediatric Surgery as the control group. Double antibodysandwich enzyme-linked immunosorbent assay (ELISA) is used to determinethe levels of MIP-1α and ECP in children with bronchiolitis in acute phase andrecovery phase and the levels of MIP-1α and ECP in the control group. Wefollow up six to twelve months after the recover of bronchiolitis and observewhether the wheezing relapse. All data is statistically analyzed by theapplication SPSS17.0.Results:1.The levels of serum MIP-1α and ECP in children with bronchiolitis ofthe acute phase were higher than those in the recovery phase and the controlgroup, the difference was statistically significant (P<0.01).2.The levels of serum MIP-1α and ECP in children with bronchiolitis ofrecovery phase were higher than the control group, the difference wasstatistically significant (P<0.05).3.The levels of serum MIP-1α and ECP in children who wheezing relapseafter the recover of bronchiolitis were higher than those who doesn’t havewheezing again, the difference was statistically significant (P<0.05).4.The level of serum MIP-1α in children with bronchiolitis in acute phasewas positive correlation with ECP, r=0.646, P<0.01.Conclusions:1. The levels of serum MIP-1α and ECP in the acute phase of bronchiolitiswere higher than those in the control group and in the convalescence stage they were dropped, but still higher than those in the control group, suggesting thatMIP-1α and ECP may play a role in the pathogenesis of bronchiolitis.2.The higher levels of serum MIP-1α and ECP in children withbronchiolitis in acute phase, the easily the occurrence of wheezing in the future.3.The level of serum MIP-1α in children with bronchiolitis in acute phasewas positive correlation with ECP.