Expression Of Cytokine In Aqueous Humor Of Rabbits With Traumatic Endophthalmitis

Endophthalmitis is a severe complication of globe-penetrating injury. traumatic endophthalmitis develop fast and visual outcome is often frustrating. It is one of the most common diseases that frequently results in loss of vision. At present, the pathogenesis of traumatic endophthalmitis is not clear. Trauma not only change the normal structure and destroy the blood-retinal barriers and cause the retinal antigen exposed. It is led to host response that contribute to the intraocular damage. But also, truma can provide a way into the eyes for bacteria. All kinds of cytokine ware produced by the uvea and the white cells were aggregated, lead to secondary inflammation.Cytokine is a kind of peptide or glycoprotein, it is produced by immunocyte and it play a regulatory role in cell function. Cytokine play a important role in endophthalmitis. But the pathogenesis is not clear. In this study, animal models of traumatic endophalmitis were established in rabbits.To investigate pathogenesis of endophthalmitis by analyzing the concentrations of interferon-y (IFN-γ),interleukin-6(IL-6),IL-8and transforming growth factor-(3(TGF-β) in aqueous humors of rabbits with traumatic endophthalmitis.Materials and MethodsA total of18rabbits were divided into three groups.Each one has6rabbits.Group one was the normal group, Animal models of traumatic endophalmitis were established in rabbits of Group two and Group three by penetrating incision at the pars plana. And staphylococcus were injected into the vitreous cavity of traumatic eyes of Group three. ELISA assay was applied to detect the levels of IFN-y,IL-6,IL-8and TGF-β in the aqueous humor before establishing the models,at6hours,12hours,24hours,48hours,96hours after establishing the models. Bacteria in vitreous cavity were couted at6hours,12hours,24hours,48hours,96hours after stablishing the models in three groups. Clinical observation was done at6hours,12hours,24hours,48hours,96hours,8days,16days after establishing the models with slit lamp and indirect ophthalmofundoscope and evaluated with clinical grading system. Rabbits kept indoors, at room temperature20℃-25℃,12/12circadian rhythm.Results1Clinical observation at different times after establishing the modelsNo inflammation in Group one. The inflammation in Group two was markedly lighter than Group three. There existed significant difference between Group two and Group three.2Expression of cytokine in aqueous humorThe levels of IFN-y in the aqueous humor at24hours after establishing the models in Group two and Group three reached their peak, they were (516.45±20.80) pg/ml and (508.21±31.45) pg/ml, markedly higher than the levels of before establishing the models.The levels of IL-6in the aqueous humor24hours after establishing the models in Group two and Group three reached their peak. They were (61.69±2.11) pg/ml and(61.81±2.18)pg/ml, markedly higher than the levels of before establishing the models.The levels of IL-8in the aqueous humor at48hours after establishing the models in Group two and Group three reached their peak. They ware (66.78±2.00) pg/ml and (64.94±3.39) pg/ml,markedly higher than the levels of before establishing the models. The levels of TGF-(3in the aqueous humor at24hours after establishing the models in Group two and Group three were (288.79±7.17) pg/ml and (293.99±1.93) pg/ml, they were markedly lower than the levels of before establishing the models.3counting of bacteriaThe bacterial culture was negative for Group one at different times, Group two at different times and Group three at96hours after establishing the models. It was positive in Group three (100%,6hours to48hours after establishing the models).Conclusions1There is no bacterium growth on agar plate at96hours after establishing the models. But the inflammation is still going on.It is concerned with the express of the changes for cytokine.2traumatic endophthalmitis led to destruction of body’s immune system and the express of the changes for cytokine. Increasing production of pro-inflammatory factors for example IFN-γ、 IL-6、 IL-8.And preventing production of anti-inflammatory factors for example TGF-β. So we should use antibiotics combined immunosuppressive agents to control Inflammation at early stage after injury.