Isolation And Purification Of Active Substances In Cotoneaster Multiflorus Bunge And Preliminary Study Of Antibiotic Activities In The Nostoc Commune

Cotoneaster multiflorus Bunge is a shrub and a kind of famous scenic bush in Rosaceae. So far, its natural products and medicinal value have not been studied. Therefore, we chose the fruitbody as material for the first time to study the pharmaceutical chemistry in order to form a basic work in the future exploration and application. In this study, we further isolate and purify the biological active substances in the crude of coarse extraction from Cotoneaster multiflorus Bunge fruitbody. The nine fractions (Fractionl--Fraction9) of the extracts of acetate phase with antioxidant and anticancer capability was collected. And Fraction2-Fraction5was used to continue to purify for their relatively higher biological activity. Finally,35compounds were gained and11compounds of them were confirmed by TLC and related spectrogram (EIMS,’H NMR,13C NMR,’H-’H COSY and DEPT). They are dibutyl phthalate, diisobutyl phthalate,1-octacosanol, sitosterol, ferulic acid, palmitic acid, oleanolic acid, uraolicacid, dipterocarpol, dammarenediol II, friedelin. Among them, steroid and terpenoids are the most parts. The antioxidant and anticancer activities of ferulic acid and oleanolic acid had been reported. Uraolicacid had also been found for its considerable anticancer capability. In addition, it had been reported the antimicrobial activity of friedelin. However, the main bioactive substances of coarse extracts still need to further study.Others, we explored the analytic methods of antibacterial and antifungi capabilities of crude extracts in Nostoc commune. The results showed that the extracts of petroleum ether and ethyl acetate had marked antibacterial capability and showed dose-dependent. At200μg/mL, the inhibition rate of extracts of petroleum ether and ethyl acetate to Staphylococcus was about70.87%and64.24%, respectively. The inhibition rate of them to bacillus was60.25%and71.58%, respectively. And the inhibition rate of them to E.coli was41.78%and44.86%, respectively. On the other hand, only the extracts of petroleum ether and ethyl acetate culturing in nutrition agar medium could form a transparent inhibition zone and also showed dose-dependent. The inhibition zone of100μg extracts of petroleum ether and ethyl acetate phase to Staphylococcus plate (22.33mm inhibition zone of20μg Streptomycin) was13.60mm and11.20mm, respectively. The inhibition zone of them to Bacillus subtilis (25.17mm inhibition zone of20μg Streptomycin) was12.00mm and9.07mm, respectively. And the inhibition zone of them to E.coli (22.13mm inhibition zone of20μg Streptomycin) was12.20mm and9.80mm, respectively. These data were similar to those in the liquid medium culture. That means the antimicrobial activity of the drugs was accurately reflected by the apparent screening and quantitative evidence. However, all of the coarse extracts were almost no obvious antifungal activities.