Expression And Clinical Significance Of MMP-9and SOX2in Human Giant Cell Tumor Of Bone

Objective：Giant celltumorofbone(GCT)is oneofthe primarybonetumorswhichhave strong invasiveness,poorclinical therapeutic response,sarcomatous change after radiation therapy,high recurrence rate and the tendency of pulmonary metastasis.The chief histology of giant cell tumor ismany well‐distributed multinucleated giant cells which suffusion and hyperplasia can findon the background of stromal cells.Pathologic grading can’tpredictthetumor’s biologic behavior correctly, which bring problems, such as correct diagosis and treatment.So it is importanttoidentifyatumorspceific makerwhich is importantinthedesignofan interventional procedure,guiding for clinical decisions and judging prognosis.Therefore, this experiment to study the roles of MMP‐9and SOX2in developmentand progressionofbonetumors.Tostudytheexpressionlevelsof MMP‐9and SOX2ingiant celltumorofboneanddetcetthe correlationbetweentheir expression levels and the histological grade, recurrence state ofthetumor.Tointendtofind possible prognostic indicationsoftheaggressive behavioroftheunderlying pathology.Materials andmethods：23cases of bone giant cell tumor fromJanuary2004to March2010in theOrthopeadieDepartmentofthe First Hospitalof Jilin University are taken for study, among which, there are11cases recurrent tumor,12cases of non‐recurrent, and9male and14female patients aged15years to71years old (average age35years). Thetumor tissues are embedded in paraffin with40g/L paraformaldehyde,buried by paraffin and cut as4um serial sections. The expression of MMP‐9,SOX2in Giant cell tumors of bone and its changes innon‐recurrent tumor group and the recurrent tumor group are observed byimmunohistochemistry.Results：1.The positive expression rate of MMP‐9in giant cell tumor of bone was52.71%.Therewerenorelevancebetweenthe expressionof MMP‐9andgender, age, invasiveloeation, Jaffegrade(P>0.05),buttheexpressionof MMP‐9wasrelevantto prognosis(P<0.05).Expressionof MMP‐9wassignificantlyhigheringian cell tumorofbonewithrecurrencethanthatingiant celltumorofbonewithoutrecurrence.2.Thepositive expression rate of SOX2in giant cell tumor of bone was56.52%.Therewerenorelevancebetweenthe expressionof SOX2andgender, age, invasiveloeation, Jaffegrade and prognosis (P>0.05).3.Therewas no significant correlationbetweenexpressionof MMP‐9and SOX2(P>0.05).Conclusions： MMP‐9and SOX2were both highly expressed in the giant celltumor of bone.Therewassignificant correlation betweenexpressionof MMP‐9and prognosis.Theoccurrencethat expressthatthe MMP‐9may participatesGCTevolve,andaffectsthemalignantdegreeandthe prognosis progressions, which canbeusedasthenewmarkingthingsoftumortodiagnoseGCTqualitatively.