The Expression And Relevance Of HPV1Protein And Tumor Suppressor Gene P16INK4A In Cervical Lesions

Objective:Research the expression and relevance of Human papillomavirus L1capsid protein and Tumor suppressor gene P16INK4Ain cervical lesions. Methods Chose210cases of HPV (+) patients in shanxi Cancer Hospital including Mild inflammation30cases、ASCUS50cases、LISL50cases、HISL50cases、SCC30cases, among them, inflammation37cases、CINⅠ53cases、CINⅡ42cases、 CINⅢ50cases、Cervical cancer28cases,these cases have not been treated. The expression of HPVL1capsid protein and P16protein were detected by Maxision immunohistochemistry method.Results:1、The compartion of the rates of Human papillomavirus L1capsid protein expression in all levels of liquid-based cytology.The positive rate in Mild inflammation is10%(3/30)、in ASCUS is38%(19/50)、in LSIL is68%(34/50)、in HSIL is6%(3/50)、in SCC is0%(0/30), which were statistically significant (P<0.005).The couple of LSIL has the highest positive rates.The positive location is in cytoplasm or nucleus of exfoliated cells.2、The compartion of the rates of Human papillomavirus L1capsid protein expression in all levels of organitations of cervix. The positive rate in mild inflammation is13.5%(5/37)、in CINⅠ is60.4%(32/53)、in CINⅡ is28.6%(12/42)、 in CINⅢ is10%(5/50)、in cervical cancer is0%(0/30), which were statistically significant(P<0.005).The couple of CINⅠ has the highest positive rates. The positive location is in cytoplasm of koilocytes.3、The compartion of the rates of P16expression in all levels of liquid-based cytology. The positive rate in Mild inflammation is13.3%(4/30)、in ASCUS is28%(14/50)、in LSIL is52%(26/50)、in HSIL is100%(50/50)、in SCC is100%(30/30. The positive rate is gradually increased by the raise of grade. The positive location is in cytoplasm or nucleus of the shaped or cancer cells.4、The compartion of the rates of P16expression in all levels of organitations of cervix. The positive rate in mild inflammation is16.2%(6/37)、in CINⅠ is22.6%(12/53)、in CINⅡ is59.5%(25/42)、in CINⅢ is100%(42/42)、in cervical cancer is100%(28/28). The positive rate is gradually increased by the raise of grade. The positive location is in cytoplasm or nucleus of the cell of CINⅢ or cervical cancer.5、The compartion of the rates of Human papillomavirus L1capsid protein and of P16expression in all levels of liquid-based cytology.The positive rate of HPV(+)P16(-) in LSIL is the highest,which is32%(16/50). The rate of HPV(-)P16(+) in HSIL、SCC respectively is94%(47/50)、100%(30/30), which are apparently higher then other couples.6、The compartion of the rates of Human papillomavirus L1capsid protein and P16expression in all levels of organitations of cervix. The positive rate of HPVL1(+)P16(-) in CINⅠ is45.3%(24/53),which is the highest in all couples. The rate of HPV(-)P16(+) in HSIL、SCC respectively is90%(45/50)、100%(30/30), which are apparently higher then other couples.Conclusion:The joint detection of the expression of Human papillomavirus L1capsid protein and P16can separate different levels of cervical lesions,the detection in specimen of liquid-based cytology can simplify the diagnostic process.