Biologically Active Components Of Medicinal Plants Potentilla Discolor Bunge

Potentilla discolor Bunge，one of the plant of the genus is Potentilla （Rosaceae）is one of the very important and commonly used herbs in Chinese medicine. Previousstudies on this plant led to the isolation of flavonoids, triterpenoids, polyphenols, tannin,and saponin compounds. Research shows that potentilla discolor bunge has enoughtrace contents which were needed by diabetes patients, the aqueous extract and ethanolextract of Potentilla discolor Bunge have a good antioxidant and hypoglycemic effects.But there is no report about the hypoglycemic effect of the constituents andhypoglycemic mechanism from Potentilla discolor Bunge. To find new effective fromthis plant, phytochemistry studies were carrying out on the extract of this plant.This subject consists of three parts: First, chemical constituents were isolated byrepeated column chromatographies, including Silica gel, Toyopearl HW-40C andpreparative HPLC. The structures of the isolated compounds were elucidated on thebasis of spectral data analysis. Second, using polyamide purification process, the totalflavonoids of Potentilla discolor Bunge was acumulated. The anti-diabieticbioactivities on the total flavonoids, the methanol extract and ethanol parts ofPotentilla discolor Bunge were screened by animal pharmacodynamics. The separatedtotal flavonoids was further separated in order to find effective constituents from theplant. Third, the room temperature ionic liquid [Bmim]BF₄was used to separateeffective constituents from the MeOH extract of Potentilla discolor Bunge.Using the repeated column chromatographies method, six compounds were isolatedfrom Potentilla discolor Bunge, pomolic acid （1）,3-O-acetyl-pomolic acid（2）,oleanolic acid（3）,2α-hydroxyl-betulinic acid （4）, quercetin-3-O-β-D-glucoside（5）,kaempferol-3-O-β-D-glucoside（6）. Compounds2and4were firstly separated fromPotentilla discolor Bunge.Six flavonoids compounds were isolated from the total flavonoid of P. dscolorBunge, kaempferol-3-O-α-L-arabinofuranoside （7）, kaempferol-3-O-β-D-glucoside（8）, quercetin-3-O-β-D-glucoside（9）,quercetin-3-O-α-L-arabinofuranoside（10）, kaempferol-3-O-β-D-（6-O-cis-p-coumaroyl）-glucopy-ranoside（11）, kaempferol-3-O-β-D-（6-O-trans-p-coumaroyl）-glucopy-ranoside（12）. Compounds7,9,11-12were firstly separatedfrom the plant.With the assisted extraction of room temperature ionic liquid [Bmim]BF₄, atriterpenoid compound, Δ²,4β-hydroxy-（25R）-methyl-5α,14α-helix-steroidal ene wasisolated from Potentilla discolor Bunge methanol parts.This subject studied the chemical composition of Potentilla discolor Bunge, andexplored the application of room temperature ionic liquids in plant chemical separation.