| Objective: Applying a tradition method basis on the morphology classification, count and measure the main morphology properties of six kinds of eel, be tied in the actual product place of eel seedling, compare by the relevance document that eel kind distributes, and identify that six groups of eel seedlings on the morphology. Use the random amplified polymorphic DNA technology to analyze the polymorphism in eel’s geneome DNA, and establish the classification and inheriting relation on molecular biology of these six kinds of eel.Method: Collect the kinds of eel that related, be divided into groups and being numbered according to place of production, use the electron analytical balance weighing, placing the eel seedling flat on the flat, use caliper to measure the eel seedling the overall length, the head length, and eye spacing directly. After measuring the fore- base distance between the dorsal fin and the anal fin, press and make it into a transparent water closing glass plate, and measure general spine number by observing under the microscope. Record the weight, the overall length, the head length, the eye spacing, the fore- base distance between the dorsal fin and the anal fin, the general spine number, and compare the statistical data and analysis. Being tied in the actual place of production eel seedling, compare by the relevance document about eel kinds distributes, and identify that six groups of eel seedlings on the morphology.Method: Clip fishtail, extract six kinds of eel organization geneome DNA, and try to find out the best condition of the Polymerase Chain Reaction system. 32 random primers were adopted to analyze the polymorphism in RAPD-PCR profiles. The outcome is passed though by agarose Gel Electrophoresis to preparat for collecting the illustrative plates of DNA diversification. Establishes the method of classify these six kinds of eel by analysis the DNA diversification on molecular biology, at the same time, carrying out statistics the data about the strip expanding, and calculating the inherit distance and similarity of six kinds of eel.Results: According to morphology of six groups of eel seedlings: Spine quantity , long fin type and caudal fin fan are circular, the distance between the dorsal fin and the anal fin fore-end base, body strong point, weight, strong point, body height, eye spacing etc., be tied in the actual product place of eel seedling, compare by the relevance document that eel kind distributes written by Tony Robinet, identify that six groups of eel seedlings on the morphology. The production place of Japan group eel is in the Northern Hemisphere of Pacific Ocean, near to Japan, a long fin type, the number of spine is 116.15±0.62, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 7.12~9.08% , identify that for Anguilla japonica; Group of Anguilla anguilla place of production is along the coast in Atlantic Ocean Europe, a long fin type, the spine number is 115.90±0.34, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 10.6 ~ 11.3%, identify that for Anguilla anguilla; Group of America eel place of production is along the coast in Atlantic Ocean North America, a long fin type, the spine number is 103~110, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 7.8~8.2% , identifies that for Anguilla rostrata; Group of Pacific Ocean eel place of production for the equator vicinity Pacific Ocean, a long fin type, the spine number is 104.27±0.65, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 12.8~15.2% , identify that for Anguilla mormorata; Group of South Africa eel place of production is along the coast in Indian Ocean South Africa east, a long fin type, the spine number is 103.37±0.35, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 13.5~14.4%, identifies that for Anguilla mossambica; Group of the Philippines eel place of production is along the coast in the Philippines, a long fin type, the spine number is 104.09±0.62, the distance between the dorsal fin and the anal fin fore-end base of the overall length accounts for 9.27~10.7% , identify that for Anguilla celebesensis.The outcome is passed though by agarose Gel Electrophoresis to sift out 13 random primers that increasing the production of fairly good stretching of simultaneous limpid, much morphology, this 13 use together increasing comeout 129 strips belt, range of fragment big or small is 250~2500bp. The strip belt that Anguilla mossambica and Anguilla celebesensis among them produce has been the most, 28 strips belt have appeared , the group creation strip belt number has been 25 strips belt other in proper order spend an eel, 20 has brought the Anguilla japonica along, Anguilla rostrata 17 strips belt, Anguilla anguilla 11 strips belt. Attractly, primers S21 among them is a molecular mark of six kinds of eel interspecific crossing alone. Carry out inheritance on the strip belt gaining according to formula from when analytical, the modulus counting the interspecific crossing similarity getting six kinds eels is at a distance of with inheritance: The Anguilla mossambica and the Anguilla japonica similarity modulus are 0.4762, with the Anguilla mormorata similarity modulus is 0.4905,The modulus is 0.1952 with Anguilla rostrata similarity, the modulus is 0.2262 with Anguilla anguilla similarity , the modulus is 0.2524 with Anguilla celebesensis similarity; The Anguilla japonica and the Anguilla mormorata similarity modulus are 0.4333, the modulus is 0.2738 with Anguilla rostrata similarity, the modulus is 0.3333 with Anguilla anguilla similarity , the modulus is 0.2833 with Anguilla celebesensis similarity; The Anguilla mormorata and the Anguilla rostrata similarity modulus are 0.2429 , the modulus is 0.2667 with Anguilla anguilla similarity , the modulus is 0.4262 with Anguilla celebesensis similarity; The Anguilla rostrata and the Anguilla anguilla similarity modulus are 0.5833 and the Anguilla celebesensis similarity modulus is 0.2976; The Anguilla anguilla and the Anguilla celebesensis similarity modulus are 0.1595. Will inherit the distance experiment data entry computer, the clustering being in progress with NTSYS software analyses, adopt UPGMA to follow the affinity relation picture structuring six eel breeds. The Anguilla anguilla and Anguilla rostrata genetic relationship clustering analysis is demonstrated are nearest , the primo is gathered being of the same class; But the Anguilla mormorata and the Anguilla mossambica have closer genetic relationship , the Anguilla japonica and that approach also comparatively, Anguilla celebesensis relation is slightly distant , this 4 kinds eels get together to be of the same class. Result explains that the eel genetic relationship and the eel geographical distribution go hand in hand , may accept the effect that ocean current moves except being affected by the geographical distribution. |
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