Study On High Quality Of Penniseetum By Morphological And RAPD Marker

Penniseetum is quality pasture resources, plant are very tall and its varieties yield and nutritional quality is better. Moreover, with the depth of research, people are more and more familiar with other aspects of the application gradually .Therefore, the introduction of quality Pennisetum and Breeding has become more important than ever. As a result of Pennisetum is intra inter species-hybrid repeatedly, and the breeding parents also relatively concentrated, Variety of morphological difference is getting smaller and smaller,caused morphological identification of Pennisetum quality pasture getting difficult . The reason is the variety happen the larger genetic change. This paper, we study 11 different varieties of quality Pennisetum from Zhongkai univercity of Agriculture technology planted .Built a response system and procedure of RAPD reaction suitable for Pennisetum and comparative study Pennisetum species identification by morphological identification and RAPD markers. After variance analysis and cluster analysis to explore genetic variation about their relationship between the level of molecules and morphology . The main research results are as follows :The 11 penniseetum cuttings grown after 18 mounth, There are ninie important morphological characteristics of 11 Penniseetums were analyzed: Natural high, Absolute height,Internode length, Stem height, Minister for axis and short axis, The number of burl, leaves length, leaves width. The results indicated that there was relatively high genetic diversity in 11 Penniseetums, the coefficient of variation between 22%-43%.Through the orthogonal design choose the experimental procedure and optimize the proxedure.We found the best concentration of Mg²⁺ ,dNTPs,Taq and primer. The reaction system was: In a total volume of 20ul, consisting 1μL 10×buffer, 2.0mmol/L Mg²⁺, 0.75 U Taq DNA polymerase, 150μmol/L dNTPs, 0.50μmol/L primer, 25ng of template DNA.The optimal RAPD-PCR reaction conditions for Penniseetum were set up. Amplification was performed under the following cycle profile: 2min at 94℃followed by 40cycles of 20s at 94℃, 30s at 37℃, and 90s at 72℃, and one cycle of 10min at 72℃for final extension, hold in 4℃ From 50 radom primer, There are eight primers were chosen and used to analyse 11 Penniseetum. A total of 60 out of 82 amplification products were polymorphic, percentage of polymorphic loci was73.2%. Percent polymorphism varied form 57.1-85.7 among the polymorphic primers. Number of amplification products ranged form 7 to 14, with an average of 10.The clustering pattern was obtained by the analysis of morphological characteristics and RAPD of Penniseetum. The results indicated that morphological characteristics not showed strict relationship with RAPD marker. In the morphological characteristics analysis has the sample deviation in their exist population, is incompletely consistent with the RAPD mark . the reason of situation is various, on the one hand the morphology characteristics influence by the environment, even if is the close varieties, their best habitat possibly completely is also inconsistent; On the other hand Penniseetum breeding parent relative centralized, the variety the morphology difference becomere much smaller ,and difficulty distinguished from the morphology. The morphology and the molecular level RAPD analysis have much difference ,the reason of that will await for the further research.