The Study Of Forsythiaside On Antioxidant And Anti-inflammatory Activity Of Liver In Chickens

Forsythia is a traditional medicine of China, the clinical application is very extensive, curativeeffect and side effect is low, and the biological activities are various. Forsythiaside is thephenylethyl alcohol glycosides extracted from Forsythia. It is one of the main effective componentsdevelop pharmacological activitiesst, such as antibacterial, antiviral, antioxidation. At present, themain of coverage of forsythia ester glucoside in resistance to bacterial and viral infectionsantioxidant and immune regulation. It is still unclear how forsythiaside regulate inflammatoryresponses in liver of chicken.The liver is the most concentrated place of the monocyte-macrophagecell system in the body. It is also the main organs to clear endotoxin. So the liver becomes one ofthe most sensitive organs in the systemic inflammatory response induced by LPS. Therefore,searching for effective drugs to reduce liver damage caused by LPS to prevent and treat liverdiseases and LPS multiple organ failure has important clinical value. Therefore, this study wasconducted to investigate the effects of forsythiaside on inflammation in chicken challenged withLPS and discussed the possible mechanism, to provide theoretical basis for the further research andclinical application of forsythiaside.Firstly, the study was conducted to investigate the effects of forsythiaside on antioxidant inchicken liver after challenging with LPS. The chickens were divided into groups of high, low-dosewhich were treated by forsythoside respectively, the normal control group and LPS control group.The whole liver was removed, the levels of superoxide dismutase (SOD), total antioxidationcapacity (T-AOC), Malondialdehyde (MDA) and glutathione (GSH) were determined respectively.These results suggest that the groups of by forsythiaside (30,60mg/kg) increased (P<0.05) thevitality of SOD, T-AOC and decreased the content of MDA (P<0.05) compared with LPS controlgroup. Consequently, oral administration of forsythiaside could significantly decrease oxidationstress, suppress lipid peroxidation and improve antioxidant capacity.Secondly, this test was used Griss method and RT-PCR to detect the content of NO and iNOSmRNA expression level in chicken liver respectively.The results show that, the data indicates thattreating with forsythiaside had significantly inhibits LPS-induced the production of NO andexpression of iNOS mRNA compared with the LPS group. The reduced NO production byforsythiaside may be suppressed expression of iNOS which is the key enzyme responsible for NOproduction under LPS-induced liver inflammation. Thirdly, this test was used ELISA and RT-PCR methods to detect the content of IL-1β, IL-6and IL-17in chicken liver respectively. Injection with LPS increased (P<0.05) the content of IL-1β,IL-6and IL-17compared with the control group. The groups of forsythiaside (30,60mg/kg)decreased (P<0.05) the protein and mRNA levels of IL-1β, IL-6, TNF-α and IL-17compared LPScontrol group.These results suggest that oral administration of forsythiaside mitigated the liverinflammatory response induced by LPS through changing the production of pro-inflammatorycytokines such as IL-17, IL-1β, IL-6, and iNOS.In one word, the study shows that forsythiaside had significantly inhibits LPS-inducedinflammatory responses in chickens, some of its relationship between inflammatory mediators andantioxidant are expounded, to provide the theoretical basis for the further research and clinicalapplication of liver injury caused by inflammation of forsythiaside.