Effects Of Benzoic Acid And Guanylacetic Acid On Growth Performance,Anti Inflammatory And Antioxidant Capacity Of Broilers

The purpose of this study is to explore the effects of benzoic acid and guanidinoacetic acid on the growth performance,anti-inflammatory and antioxidant capacity of broilers,and to provide experimental basis for their application in broiler breeding.This study included two trials: Experiment 1.Effects of benzoic acid on growth performance,anti-inflammatory and anti-oxidative capacity of broiler chickens.Using a single-factor completely randomized design,144 1-day-old AA broiler chickens were selected and randomly divided into 4 groups,with 6 replicate cages in each group and 6 chickens in each replicate cage,fed with basal diet(control group)and supplementary diet respectively 70(H70),105(H105)and 140 mg/kg(H140)benzoic acid were tested in the diet,and the test period was 6 weeks.The results showed that:(1)Benzoic acid significantly affected the daily gain and feed conversion ratio of1-21 d and 22-42 d broilers(P<0.05),and the daily gain of the H105 group was significantly higher than that of the benzoic acid blank control group from 1-21d(P<0.05),and the feed conversion ratio of the benzoic acid experimental group was significantly lower than that of the benzoic acid blank control group(P<0.05);the daily weight gain of the H105 and H140 groups was significantly higher than that of the benzoic acid blank control group on 22-42d(P<0.05).The feed conversion ratio of the three groups in the formic acid experimental group was significantly lower than that in the benzoic acid blank control group(P<0.05).(2)Benzoic acid significantly affected the levels of IL1β and IL6 in serum of broilers from 1-21 d and22-42d(P<0.05),and significantly affected the levels of IL10 and TNF-α on 22-42d(P<0.05),among which 1-The levels of serum IL1β and IL6 in the three groups of benzoic acid test groups on 21 d and 22-42 d were significantly lower than those in the benzoic acid blank control group(P<0.05);the levels of IL10 in the H105 and H140 groups on 22-42 d were significantly higher than those in the benzoic acid blank control group(P<0.05).The TNF-α levels of the three groups in the benzoic acid experimental group were significantly lower than those in the benzoic acid blank control group at 22-42 days(P<0.05).(3)Benzoic acid significantly affected the levels of glutathione peroxidase,total superoxide dismutase and malondialdehyde in broiler chicken serum from 1-21 d and 22-42d(P<0.05),and the 1-21 d benzoic acid test The levels of glutathione peroxidase in the three groups of the treatment group were significantly higher than those in the benzoic acid blank control group(P<0.05),and the total superoxide dismutase level in the H70 group was significantly higher than that in the benzoic acid blank control group and other experimental groups(P<0.05).The level of malondialdehyde in the H140 group was significantly lower than that in the benzoic acid blank control group(P<0.05);the levels of glutathione peroxidase in the H105 and H140 groups from 22 to 42 days were significantly higher than those in the benzoic acid blank control group(P<0.05).The level of oxide dismutase was significantly higher than that of the benzoic acid blank control group(P<0.05),and the levels of malondialdehyde in the three groups of the benzoic acid test group were significantly lower than those of the benzoic acid blank control group(P<0.05).(4)Benzoic acid significantly affected the serum urea nitrogen level of broilers on 1-21 d and 22-42d(P<0.05),and the urea nitrogen levels in the three groups of benzoic acid test group were significantly lower than those in the benzoic acid blank control group on 1-21 d and 22-42d(P<0.05);in the 1-21 d stage,benzoic acid had a significant effect on serum albumin(P<0.05),and the albumin level in the H140 group was significantly lower than that in the benzoic acid blank control group(P<0.05);in the 22-42 d stage,benzoic acid had a significant effect on Serum total protein was significantly affected(P<0.05),and the total protein level of H140 group was significantly higher than that of benzoic acid blank control group.(5)Benzoic acid significantly affected the expression levels of IL1β,IL10,IL6 and TNF-α in broiler intestines(P<0.05),the expression levels of IL1β in H70 and H105 groups were significantly lower than those in the benzoic acid blank control group(P<0.05),and the expression levels of IL1β in H140 group The expression levels of IL10 in the three groups of benzoic acid experimental group were significantly higher than those in the benzoic acid blank control group(P<0.05),and the levels of IL6 in the H105 and H140 groups were significantly lower than those in the benzoic acid blank control group(P<0.05).The levels of TNF-α in the three experimental groups were significantly lower than those in the benzoic acid blank control group(P<0.05).Experiment 2.Effects of guanidinoacetic acid on growth performance,anti-inflammatory and anti-oxidative capacity of broilers.Using a single-factor completely randomized design,144 1-day-old AA broiler chickens were selected and randomly divided into 4 groups,with 6 replicate cages in each group and 6 chickens in each replicate cage,fed with basal diet(control group)and supplementary diet respectively 36(CT36),45(CT45)and 54 mg/kg(CT54)guanidinoacetic acid were tested for 6 weeks.The results showed that:(1)Guanidinoacetic acid significantly affected the daily gain of broilers on1-21 d and 22-42d(P<0.05),and the average daily gain of CT36 group on 1-21 d and22-42 d was significantly higher than that of the guanylacetic acid blank control group(P<0.05);Guanidinoacetic acid significantly affected the daily feed intake of broilers from 1 to 21 days(P<0.05),and the average daily feed intake of CT36 and CT45 groups was significantly higher than that of the guanylacetic acid blank control group(P<0.05);Guanidinoacetic acid significantly The feed conversion ratio of CT45 and CT54 groups was significantly lower than that of the guanylacetic acid blank control group(P<0.05).(2)Guanidinoacetic acid significantly affected the levels of IL1β,IL6 and IL10 in broiler chicken serum from 1-21 d and 22-42d(P<0.05)and the level of TNF-α in broiler chickens from 22-42d(P<0.05),and 1 At-21 d,the IL1β levels in the CT45 and CT54 groups were significantly lower than those in the guanylacetic acid blank control group(P<0.05),the IL10 levels in the CT36 group were significantly higher than those in the guanylacetic acid blank control group(P<0.05),and the IL6 levels in the CT54 group were significantly lower than those in the guanylacetic acid blank control group(P<0.05);The level of IL1β in the CT54 group was significantly lower than that in the guanylacetic acid blank control group(P<0.05),the IL10 index in the CT36 and CT54 groups was significantly higher than that in the guanylacetic acid blank control group(P<0.05),and the levels of IL6 and TNF-α in the three groups of the guanidinoacetic acid experimental group were significantly lower in the guanylacetic acid blank control group(P<0.05).(3)Guanidinoacetic acid significantly affected the levels of glutathione peroxidase,total superoxide dismutase and malondialdehyde in the broiler serum of 1-21 d and 22-42d(P<0.05),and the 1-21 d The levels of glutathione peroxidase in groups CT145 and CT54 were significantly higher than those in the guanylacetic acid blank control group(P<0.05),and the levels of total superoxide dismutase in groups CT36 and CT45 were significantly higher than those in the guanylacetic acid blank control group(P<0.05).The malondialdehyde level was significantly lower than that in the guanylacetic acid blank control group(P<0.05);the glutathione peroxidase level in the CT54 group was significantly higher than that in the guanylacetic acid blank control group(P<0.05)at 22-42 days,and the total superoxide dismutase in the CT36 group The levels of malondialdehyde in the three groups of the guanidinoacetic acid test group were significantly lower than those in the guanylacetic acid blank control group(P<0.05).(4)Guanidinoacetic acid significantly affected the serum total protein and serum urea nitrogen levels of broilers on 1-21 d and 22-42 d,and the total protein level of CT54 group on 1-21 d was significantly higher than that of the guanylacetic acid blank control group(P<0.05),guanidine The levels of urea nitrogen in the three groups of glycylic acid test group were significantly lower than those in the guanylacetic acid blank control group(P<0.05);the total protein level in the CT45 group at 22-42 days was significantly higher than that in the guanylacetic acid blank control group(P<0.05),and the urea nitrogen levels in the CT36 and CT45 groups The level was significantly lower than that of the guanylacetic acid blank control group(P<0.05).(5)Guanidinoacetic acid significantly affected the expression levels of IL1β,IL10,IL6 and TNF-α in broiler intestines(P<0.05),and the expression levels of IL1β in the three groups of guanidinoacetic acid test group were significantly lower than those in the guanylacetic acid blank control group(P<0.05),the expression level of IL10 gene in CT36 and CT45 group was significantly higher than that in guanylacetic acid blank control group(P<0.05),the expression level of IL6 gene in CT45 and CT54 group was significantly lower than that in guanylacetic acid blank control group(P<0.05),the three groups of guanidinoacetic acid experimental group The expression levels of TNF-α were significantly lower than those in the guanylacetic acid blank control group(P<0.05).In summary,both benzoic acid and guanidinoacetic acid can improve the growth performance,anti-inflammation and antioxidant capacity of broilers.The appropriate dosage of benzoic acid is 105 mg/kg,and the dosage of guanidinoacetic acid is 54mg/kg in 1-21 days and 22-42 d is 45mg/kg more appropriate.