Effects Of Single And Joint Exposure Of Atrazine And Chlorpyrifos On Heat Shock Protein In Common Carp (Cyprinus Carpio)

Atrazine (ATR) and chlorpyrifos (CPF) are triazine herbicides and organophosphorusinsecticides，respectively. In recent years, ATR and CPF receive more and more attention becauseof their large usage in agriculture, long residual period, highly frequency of detection in aquaticenvironment and disruption of endocrine system. As a molecular chaperone, heat shock protein(HSP) could increase the expression in response to environmental stresses, repair damaged proteinsand improve the resistence against stresses. The rapid induction of HSPs makes it an idealbiomarker for monitoring environment pollution. In the present study, the common carp wereexposed to ATR (428,42.8and4.28μg/L), CPF (116,11.6and1.16μg/L) and their mixture (113,11.3and1.13μg/L), then the protein levels and mRNA expression of HSP60, HSP70and HSP90inliver, brain, kidney, gills, head kidney and spleen were detected on the basis of preparion HSP60,HSP70and HSP90polyclonal antibodies. The results showed that:1The polyclonal antibodies were successfully prepared by using the proteins of HSP60, HSP70and HSP90to immunize the New Zealand rabbit.2HSP60expression was induced in head-kidney, spleen and brain by ATR, and in head-kidneyby CPF and CPF/ATR. In kidney, HSP60was induced in low dose groups but suppressed in highdose groups. In gill, the level of HSP60was lower than the control group (P<0.05).3The HSP70expression was induced in a dose-dependent manner in all treated groups exceptthat in CPF and mixture exposure in kidney (P<0.05).4HSP90was induced in brain, liver, spleen, head-kidney and kidney in ATR, CPF or CPF/ATRgroups, and supressed in gill. The peak level of HSP90appeared at low dose group in brain andhead-kidney. In liver and kidney，the expression of HSP90increased significantly in high dosegroups. However, the mRNA transcription in gill decreased with the increase of pesticideconcentration (P<0.05).In conclusion, the exposure of ATR and/or CPF could increase the expressions of HSPs in live,brain, head-kidney and spleen which suggested that HSPs played key roles in ATR and CPFtoxicity process. Howerer, the expressions of HSPs in gill and kidney were suppressed which alsosuggested that the HSPs expression were tissue specificity. In this study, we comfirmed that theexpression of HSP70in liver, brain, head-kidney and spleen as well as the mRNA expression ofHSP90in gill could be a suitable biomark to the exposure of ATR and/or CPF.