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The Effect Of Temperature Change On Picea Schrenkiana Autotoxicity

Posted on:2013-07-02Degree:MasterType:Thesis
Country:ChinaCandidate:S L LiFull Text:PDF
GTID:2233330395465878Subject:Ecology
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Picea schrenkiana is not only endemic species of Central Asia and the central mountain of Asia, but also is the most important vegetation of forest zone in Tianshan Mountains in Xinjiang China. With the basis of Hypotheses of temperature effect on autotoxicity, which have relation with the natural regeneration obstacle of P. schrenkiana. It is, in fact, quite clear those P. schrenkiana litters contain autotoxicity active materials, with the globle warming on earth, this paper set the topsoil(1-5cm) temperature of P. schrenkiana natural woodland(May-August) as a standard, chose the litters’ organic extract phase(ethylether, ethyl acetate,normal butanol) and the potencial Auto-toxicity,3,4-Dihydroxyacetophenone (DHAP), which is isolated from ethylether extract phase, make use of artificial intelligence to simulate the tempreture change, the study emphasizes the P. schrenkiana Autotoxicity responses to climatic change. The main achievement is follow:Temperature change has influences on autotoxicity of extract phase from P. schrenkiana litter, ethylether, ethyl acetate, normal butanol and DHAP, under a variety of temperature conditions; the autotoxicity of ethylether extract phase is much stronger than others. Meanwhile, temperature change affects on the seedling growth of P. schrenkiana, the temperature12℃and4℃, a very close approach to the natural woodland growing season diurnal temperature, is just used to raise of P. schrenkiana seedlings; however, temperature has2℃raise up(14℃-6℃) or fall down(10℃-2℃) both cause seedlings growth; and will stop growing on8℃-0℃, die or decay in the end.Temperature change has no significant effect on the strong autotoxicity of P. schrenkiana. When medium concentration of Ether extraction phase is less than or equal to1mg/mL, temperature increasing will promote the seed germination; when it is greater than or equal to2mg/mL, the condition of12℃-4℃temperature will inhibit the seed germinate. The medium concentration of ethyl acetate is greater than or equal to0.5mg/mL, the effect of temperature change on seed germination will be less than the concentration on seed germination. The degree of inhibition to seed germination gradually increased with the treatment solution concentration increasing. Under the same treatment concentration, effects of temperature on seed germination weaken with increasing temperature.The autotoxicity effect of3,4-Dihydroxyacetophenone on P. schrenkiana is largely affected by temperature change, enhanced with increasing temperature. When the concentration of treatment solution is under0.1mM and0.5mM, the different kinds of temperature inhibit P. schrenkiana seed germination. But the influence of temperature on seed germination has no significant difference (p<0.05). When the concentration of3,4-Dihydroxyacetophenone treatment solution greater than or equal to7.5mM, the temperature of14℃-6℃and16℃-8℃significantly inhibit P. schrenkiana seed germination.
Keywords/Search Tags:Picea schrenkiana, autotoxicity, 3,4-Dihydroxyacetophenone (DHAP), seed germination, seedling growth
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