Effects Of Different Dietary Energy Levels On The Expression Of FSHR And LHR Gene In Ovarian Of Sheep

Dietary energy levels is an important factor affecting the reproductive performance, but how energy to impact on the molecular mechanism of animal breeding related gene expression has not been well described. The animal’s reproductive activity is mainly affected by the regulation of the reproductive axis, most studies have confirmed that the follicle-stimulating hormone (FSH), luteinizing hormone (LH) is an important regulatory factor. Therefore, the study of influence on the gene expression and regulation of FSHR and LHR in dietary energy levels can promote understanding of the mechanism of action of FSH and LH, thereby further improve the performance of animal breeding, and provide a theoretical basis for future studies of nutrients regulation in sheep ovarian tissue. The follicle development degree of ovarian determines the reproductive performance of dams of the ovulation rate and litter size. Energy levels in the diet affect the development of the follicle, causing changes in ovulation rate and litter size. Many studies have shown that the gonadotropins of FSH and LH are the main regulator of follicular development ovulation. FSH is a follicle assembly launch and follicular atresia in the degradation of the main block, and promotes more follicles mature to the preovulatory stage; LH promotes ovulation of preovulatory follicles. At present, research exist in pigs, chickens ovarian FSHR expression, but the sheep of dietary energy levels in FSHR expression in ovarian tissue has not been reported.Choosing 36 hybrid female lambs (Dorset♂×small tailed Han sheep♀), aged 4-5 months, with similar body weights, were randomized into three groups and exposed to different dietary energy levels (7.21, 10.33 and 13.49 MJ/d of their metabolizable energy but with similar protein levels). After being fed for 40 days, the animals were slaughtered to allow sampling of their ovarian. The samples were subjected to real time quantitative PCR method to detect the expression of FSHR and LHR mRNA in ovarian tissues of sheep.The results are as follows:1. Sheep ovaries of FSHR mRNA expression is relevant with the amount of dietary energy levels: The mRNA expression of FSHR in moderate energy group was much higher than the low-energy group and the high energy group, Meanwhile the difference was highly significant (P <0.01), The mRNA expression of FSHR in high-energy group was higher than the low-energy group but the difference was not significant (P> 0.05).2. Sheep ovaries of LHR mRNA expression is relevant with the amount of dietary energy levels. The amount of LHR gene expression increased with the increase of energy levels, but the difference between the three groups was not significant (P> 0.05).3. During the testing process I found some unknown sequence of the LHR, and successfully submitted to the NCBI, and Genbank Number is JN638725.