Effects Of Different Dietary Energy Level On The Mutton Quality And Expression Of H-FABP MRNA In Different Tissue Of Sheep

Choose healthy disease-free, 4~5 months old , weight 25.4kg of hybrids lambs which were randomly divided into 3 groups of 12,including group A (low-energy group), group B(middle-energy group),group C (high-energy group). Group B was raised according to Chinese sheep raising standards (NY/T816-2004) design requirements in the diet of protein, the metabolic energy was 10.33 MJ / d, group A and group C based on middle-energy group on the reduction and increase of 30%,separately. After 37 days feeding, slaughtering sheep and taking heart, longissimus dorsi and biceps femoris muscle tissue samples, using real time quantitative PCR method to detect the expression of H-FABP mRNA in different tissues of sheep and analyze of different energy levels on the H-FABP mRNA content differences. At the same time, taking the longissimus and biceps femoris to determine the main indicators which affect the meat quality by conventional analysis methods. The results are as follows:1.The department of water, pH value, shear force and intramuscular fat content is relevant to the level of dietary energy.Compared with the low-energy group,the loss rates of water, pH value and shear force of the the middle-energy group is not significantly difference(P>0.05).The difference of the loss rates of water and pH value between the high-energy group and the middle-energy group is not significantly. The loss rates of water between the high-energy group and the low-energy group is significantly difference(P<0.01).The pH value of longissimus between the high-energy group and the low-energy group is significantly difference(P<0.05); the pH value of biceps femoris muscle between the high-energy group and the low-energy group is significantly difference(P<0.01). The shear force of longissimus of the high-energy group is significantly lower than that of the middle-energy group and the low-energy group(P<0.01); the shear force of biceps femoris muscle of the high-energy group is significantly lower than that of the low-energy group(P<0.01),and there is on difference between the high-energy group and the middle-energy group. The intramuscular fat content of longissimus and biceps femoris muscle of the low-energy group is significantly lower than that of the middle-energy group and the high-energy group(P<0.01),and the difference between the middle-energy group and the high-energy group is also significantly different(P<0.01).2.The content of H-FABP mRNA in different organism is rised as the energy level increased, it is significantly difference among the low-energy group, the middle-energy group and the high-energy group in longissimus and biceps femoris muscle(P<0.01). The content of H-FABP mRNA in cardiac tissue of the high-energy group is significantly higher than that of the low-energy group and the middle-energy group(P<0.01); it is significantly difference between the low-energy group and the middle-energy group(P<0.05)3.The content of H-FABP mRNA is variability in different organism. The content of H-FABP mRNA is not significant difference in longissimus and biceps femoris muscle(P>0.05),but the content in cardiac tissue is significantly higher than in longissimus and biceps femoris muscle(P<0.01).