Study Of β-catenin Gene Clone And Location In Skin And Skin-sensor Of Phrynocephalus Prewalskii

This study focuses on the research that morphological structure and the level of β-Catenin gene expressed in skin and skin-sensor of Phrynocephalus przewalskii.We have carried out into research, expression level and conservation analysis of β-Catenin gene in different species, which would help us to elucidate the role of β-Catenin gene in skin-sensor development of Phrynocephalus przewalskii. Method:H&E staining, Immunohistochemistry, In situ hybridization.Results showed that:(1) The skin of Phrynocephalus przewalskii has five layers, which is consist of oberhautchen layer, β-layer, mesos layer, a-layer and germinal layer sequentially.(2) Tactile corpuscles exist in the concave of the skin sensor of Phrynocephalus przewalskii.(3) There are pigment cells in the the skin-sensor of Phrynocephalus przewalskii cap-like protrusions, the pigment particles are mainly distributed in the pigment cells near the free surface.(4) The immunohistochemistry results show that β-Catenin expressed in cell matrix of germinal layer.(5) In situ hybridization results show that β-Catenin abundantly expressed in germinal layer, but unexpressed in tactile corpuscle, when Phrynocephalus przewalskii is in molting period or wound healing period.In summary, this study aimed to elucidate the role of P-Catenin, which involved in skin development among different species, by researching the expression of β-Catenin in skin or skin-sensor of Phrynocephalus przewalskii. Further more, to elucidate the mechanism how β-Catenin plays an important role in the development during hair follicle differentiation, which will help us to understand the role of B-Catenin gene in Phrynocephalus przewalskii skin development and evolution.