Influence Of Climatic Factors On Genetic Diversity Of Dipus Sagitta Pallas

Dipus sagitta widely distributed in mengxin district,is a typical species in mengxin district. Mitochondrial DNA sequence of cyothrome b gene from9populations were evaluated the relationship between genetic diversity and climatic factors,investigated the genetic structure. Using molecular ecology software to analysis the sequence information. The research results obtained are as follows:1. Dipus sagitta cytochrome b nucleotide sequence nucleotide content:T(U)29.1%,C30.4%, A26.2%,G14.3%.12transition sites and1transversion site among the1137base pairs of cytb transition sites:transversion sites12:1,7conversion sites in TC,5conversion sites in AG.2. Dipus sagitta have the nucleotide diversity0.018±0.025, haplotype diversity0.963±0.054. The result show that the population of Dipus sagitta has a high genetic diversity. Dipus sagitta populations nucleotide diversity is highest in AKS(π=0.0733), the lowest in ZQZ(π=0.0010). haplotype diversity is lowest in JT population (h=0.833).3. Dipus sagitta9populations have found64haplotypes in78samples.9haplotypes in ZQZ,15haplotypes in JMN,3haplotypes in AKS,9haplotypes in AX,12haplotypes in MQ,6haplotypes in SGH,6haplotypes in GT,3haplotypes in JT,2haplotypes in YZ.AX and AKS MQ share a same haplotype. AX and SGH shared1same haplotyoe. Between the other geographic populations no shared haplotypes based on the cytb sequence.4. The correlation between nucleotide diversity and VCT was a significant negative. VCT were the dominant factors that influenced the genetic diversity of Dipus sagitta.5. In nine geographic populations of Dipus sagitta,the population of JMN and YZ genetic diversity Fst is the highest0.97518. The geographic populations of AX and YZ genetic diversity Fst is the lowest0.00752. The genetic diversity between populations is significant.6.Genetic distance between JMN and ZQZ reach the maximum0.132. Compare with other populations,we found ZQZ and JMN geographical distance also is the furthest,1579km. 7. Construted bayesian molecular phylogenetic tree, the results show that nine populations clustered into two major lineage.Other geographical populations as a branch, JMN as one branch. On the basis of BEAST software,the two branch divaricated in0.052Ma years ago. Population demography analysis indicated the Dipus sagitta experienced expansion during the past0.115Ma,which indicated that interglacial period might have effect on Dipus sagitta populations.