Research On Expression Of Heat Shock Protein Family In Callus Of Healing Mice

Heat shock proteins (HSPs) are a group of specific proteins, which express inresponse to various biological stresses, to preserve cell survival under adverseenvironmental conditions. HSPs function primarily as molecular chaperones,facilitating the folding of other cellular proteins, preventing protein aggregation, ortargeting improperly folded proteins to specific pathways for degradation.Bymodulating inflammation, wound debris clearance, cell proliferation, migration andcollagensynthesis, HSPs are essential for normal wound healing of the skin.The abnormal expression of HSPs may contribute to wound healing deficiencies,and there are differences in the roles played in wound healing of different HSPs.Furthermore, there is a lack of systematic comparison research on HSPs in woundhealing. In this study, BALB/c mice were used as the experimental animal model.3days (3d) group after wound was screened as the research object for inflammation,which3day was the most intense and the difference of which was significant (P<0.01)compared with the other time points.84heat shock protein family genes were selectedto prepare real-time quantitative PCR microarray (qPCR array). The results ofdifferential expression showed that Hsp90aa1, Hspa1a, Dnaja3were up-regulated andsignificant differences (P<0.05), Bag3, Bag4were down-regulated and significantdifferences (P<0.05).GO (Gene Ontology) analysis of differentially expressed genes showed thatHsp90aa1might participate in inflammatory reactions through up-regulatedexpression, interact with other HSPs family factors, and be involved in cell regulationand intracellular signaling, then promote migration of fibroblasts to accelerate woundhealing; Hspa1a up-regulation inhibits the inflammatory response to ensure the correct conformation of newly synthesized proteins in the wound healing process;Dnaja3, which has two isoforms with sharply contrasting functions, is related toapoptosis, and its up-regulating significance needs further interpretation; Thedown-regulation of Bag3and Bag4genes in the early stages of wound healing maypromote apoptosis of inflammatory cells and the healing of the wound tissue.Signaling pathway hypothesized that Hspa1a gene was involved in the MAPKsignaling pathway and Hsp90aa1gene was involved in NOD-like receptor signalingpathway, which might be related to adjustment of wound healing.Western blot analysis showed that the expression of Hsp90aa1and Hspa1a at theprotein level consisted with mRNA level.This study showed that the qPCR Array could be used to detect thehigh-throughput expression of mouse heat shock protein genes, with high sensitivityand good repeatability. The major gene of heat shock protein family factor in ourstudy can provide the experimental basis for in-depth study in trauma mechanism, andthe biological pathways that they are involved in can provide the theoretical basis forseeking treatment program to promote wound healing in clinical practice, and theqPCR Array built in this study will provide a new way to systematically study thedifferential expression of heat shock proteins in different stress conditions.