| Arabidopsis thaliana as a model plant it plays an important role in plant genomic study. A series of abiotic stress, such as drought, salinity, extreme temperature, chemical toxins, oxidative stress and so on, have serious impact on plant growth and development, which seriously affected the agronomic traits of plants and become the bottleneck of agricultural development. So study and gain of genes responding to abiotic stress is very important and useful to improve plant abiotic stress resistance or tolerance. In this study, Arabidopsis T-DNA insertion mutants were selected under abiotic stresses condition such as glucose, sodium chloride, abscisic acid (ABA) in order to identify T-DNA mutant materials with resistance or sensitivity to some of the abiotic stress, and provide research materials for further analysis on gene function. The main results are as follows:1. Glucose was used for mimic drought stress, and the optimum concentration of glucose stress is 8% during arabidopsis seeds germinate,6% during seedings grow. The Arabidopsis T-DNA insertion mutant seeds germinate under 8% glucose shown that:the relative germination rate of H4, H5, H12, H15a, H38 is significantly higher than that of WT, but there is no signi ficant difference in seedings.2. The optimum concentration of NaCl stress is 200 mM during arabidopsis seeds germinate, and 100 mM during seedings grow. Arabidopsis T-DNA insertion mutant seeds germinate under 200 mM NaCl shown that:H9, H14,060 is sensitive to 200 mM NaCl while H12,950/060 is more tolerant to 200 mM NaCl; Eight days after PLA200 seedings grow under 100 mM NaCl shown that:the primary root length is longer than that of WT; the number of lateral roots is more than WT; but there is no no significant difference in the shoot fresh weight between them.3. When germinate under 7μM ABA, 10μM ABA, the germination rate of PLA200 is significant difference to the WT:In the fifth day the germination rates of PLA200 under 7μM ABA stress is 92.0% and WT is 56%; and in the fifth day the germination rate PLA200 under of 10μM ABA stress germination rate is 81.3%, while the WT was 55.3%. Eight days after PLA200 seedings grow under 10μMABA shown that: the primary root length is longer than that of WT; the number of lateral roots is more than WT; and the shoot fresh weight heavier than that of WT.4. H38, WT germinate under 1/2MS sucrose-free medium which containing 8% glucose, in the fifth day the germination rate of H38, WT is 89.2%,44.2% respectively, and there is no green cotyledons under this stress condition; while in the same concentration of mannitol stress, we can see H38 and WT has green cotyledons in the fifth day, the number of cotyledons is 32.67,18.00 respectively which shows significantly different. H38, WT germinate under 1/2MS plus 1% sucrose medium which containing 8% glucose, in the fifth day the germination rates of H38, WT is 67.7% and 32.5% respectively, and the difference is significant. While in the same concentration of mannitol both of the two genotypes show no green cotyledons, and the germination rate was not significant between the two. There is no significant difference between H38 and WT when germinate under the low concentration of glucose (3% to 6%) stress, By the way seedings stress under 6% glucose shows no significant difference between the two. The total sugar in each seed of H38, WT is 0.44μg,0.54μg. While the soluble sugar in each seed is 0.64μg,0.56μg respectively, and the difference is significant. The total sugar content in H38, WT seeds is 5.11%, 6.40%, the starch contents is 2.03%,3.09%, the soluble sugar content was 2.98%, 3.24% respectively which is significantly different. In the seeds H38 oleic acid contents is 15.27% while WT is 14.05% and linolenic acid content is 18.28%, 20.52% respectively, twenty-two carbon acid content is 1.76%,1.92% all of these are significantly different. |
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