Experimental Study On Honeybee (Apis Cerana) Electroantennogram

This thesis describes honeybee olfactory systems, including the morphography characters and the distribution of olfactory receptors, the inner neural pathway of signal transfer. Based on the physiologic characters we introduce a technology to detect the electric status of honeybee's olfactory neural system, electroantennogram (EAG). Then we briefly introduce the origin, development and current research status worldwidely on EAG. After that we explain the mechanism, the measurement and the application of EAG. Imitating the structure, function and methodology of the product of the Syntech, which company's product is the most widely selected as the EAG detection system in the world, we build our own EAG detection system. The set-up consists of four parts:two micro-manipulators, a differential pre-amplifier, a stimulus providing system and an oscilloscope. Using this set-up to record EAG more than one hundred honeybees, we obtain stable EAG and spike signal to draw some conclusions from the experimental results. We successfully train the honeybees to react to the explosive odors and 30% of them can detect explosive odor after training. After we get the trained honeybees, via comparing the EAG and spike signal obtained from trained and untrained honeybees respectively, we find different patterns between them. So there is important evidence to support that using EAG and spike signals to detect explosive is realizable.