| Objective: Hyperthyroidism is the diease that the thyroid gland itselfproduces too much thyroid hormone then causes thyrotoxicosis.Thatperformces high metabolic syndrome.It will affect the nervous system,cardiovascular system,the igestive system,skeletal muscle and reproductivesystem on the different levels.The incidence of hyperthyroidism during pregnancyis0.2%.Thyroid hormone(T4)can transmit the placenta into the fetal bloodcirculation system.Hyperthyroidism affect on both maternal and offspring,it may lead tointrauterine growth retardation,prematurebirth,stillbirth,hyper-orhypothyroidismdevelopment.The domestic and foreign research shows that hyperthyroidismincreases liver cell apoptosis.This research will explore different degrees ofhyperthyroidism matrix expressing the influence on different growth periodsof the offspring in thyroid function.Methods: Randomly divided female SD rats into normal control group(N group), mild hyperthyroidism group (group A), severe hyperthyroidismgroup (group B). To establish the rat's hyperthyroidism model withlevothyroxine sodium, then made female rats and male SD rats in one cage by2:1ratio. After conception,continued giving levothyroxine sodium to A and Bgroup,N group to the same amount of saline. Observed each group rat generalcondition as well as their postnatal rats. Tested21days and60days thyroidfunction of rats offing. Observed the0day,21days,60days histopathologicalliver by HE staining and the expression of caspase-3and Bcl-2by westernblotting technology.Result:1.General situation of pregnancy rats:The rats whose condition indrinking and eating,mental state,daily activities in control group was normal.Mild hyperthyroidism rats'drinking water quantity increased slightly and daily mental state is a little excited. Severe hyperthyroidism rats' drinking waterquantity increased significantly and were easily stimulated, presenting theobvious signs of high metabolism.2. The thyroid function examination of pregnant rats: The level of FT3and FT4of the mild hyperthyroidism group and the severe hyperthyroidismgroup was more significantly increased than normal control group (P<0.01).The level of FT3and FT4of severe hyperthyroidism group was increasedsignificantly contrasting the mild hyperthyroidism group (P<0.01). The levelof TSH of mild hyperthyroidism group and severe hyperthyroidism group wasdecreased significantly than normal control group (P<0.01).The level of TSHsevere hyperthyroidism group was decreased significantly contrasting the mildhyperthyroidism group (P<0.01).3.General situation of the postnatal rats:The postnatal rats of controlgroup grow well.The postnatal rats of the mild hyperthyroidism group and thesevere hyperthyroidism group were loss weight.4.The thyroid function examination of postnatal rats:The level of FT3and FT4in21days mild hyperthyroidism group and sever hyperthyroidismgroup was higher than normal control group (P<0.01), TSH was lower thannormal control group (P<0.01). The level of FT3and FT4in21days severhyperthyroidism group was higher than mild hyperthyroidism group (P<0.01),TSH was lower than mild hyperthyroidism group (P<0.01). The level of FT3and FT4in60days mild hyperthyroidism group was higher than normalcontrol group, TSH was lower than normal control group but both were notstatistically significant (P>0.05), The level of FT3and FT4in60days severhyperthyroidism group was higher than normal control group (P<0.01), TSHwas lower than normal control group (P<0.01). The level of FT3and FT4in60days sever hyperthyroidism group was higher than mild hyperthyroidismgroup (P<0.05), TSH was lower than mild hyperthyroidism group (P<0.05).The level of FT3and FT4in60days of mild hyperthyroidism group and severhyperthyroidism group was higher than in21days, but was not statisticallysignificant(P>0.05).The level of FT3and FT4in60days of sever hyperthyroidism group was lower than in21days, but was not statisticallysignificant(P>0.05).The level of TSH in60days of mild hyperthyroidismgroup and sever hyperthyroidism group was higher than in21days (P<0.01).5.Pathological changes in the liver of postnatal rats under Lightmicroscope:The cells of liver in the control group postnatal rat were in order.Liver lobular disorder, local necrosis of liver cell, fat assault, liver cellpigmentation was observed in the hyperthyroidism groups.6.Western blot results:The expression of caspase-3protein in the mildhyperthyroidism group and the severe hyperthyroidism group of21day wasobviously increased than in the control group(P<0.01). There had nodifference between the mild hyperthyroidism group and the control group of60day(P>0.05). The expression of caspase-3protein of60day in the severehyperthyroidism group increased than the control group(P<0.01).7.The correlation analysis:The caspase-3of postnatal rats had the positivecorrelation with FT3and FT4,the negative correlation with TSH,but it had nosatistical differences(P>0.05). The caspase-3of postnatal rats had the positivecorrelation with FT3of pregnant rats(P<0.01). The Bcl-2of postnatal rats hadthe positive correlation with FT3and FT4,the negative correlation withTSH,but it had no satistical differences(P>0.05).It had the positive correlationwith the TSH of the pregnant rats(P<0.05).Conclusion:1.Thyroid hormone can through the placenta into the fetal bloodcirculation, resulting in neonatal rat hyperthyroidism.The more seriousdegree matrix hyperthyroidism, the more obvious degree hyperthyroidism ofrat offspring.2.The experimental research shows that hyperthyroidism can cause liverapoptosis,with caspase-3increasing and Bcl-2decreasing.Too much thyroidhormone breaks the balance of apoptosis, activating the executive protease.3.Severe hyperthyroidism influence on thyroid function of offspring isseriously.No matter the symptom and physical sign,also tissue pathologyexpression,it has the obvious difference with the normal group. |
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