The Effect And Mechanism Of Ginsenoside Rg1Alleviating Renal Lesion In Rats With Nephrotic Syndrome And Kidney-Qj Deficiency

Objectives:1. To investigate the effects of Ginsenoside Rgl on serum biochemical index, renal function, and renal pathology in rats with nephropathy.2.To detect the rats' serumlevel of IL-4and TNF-α and explor the ginsenoside Rgl's protective mechanism to kidney.3.To study the correlation relationship between ginseng tonifying primordial Qi and regulating inflammatory factors.Methods:Random samplled eight rats for normal group from Forty male SD rats, the other were injected adriamycin(5mg/kg) in caudal vein once,and be hang the tail twice every day to reinforce the Kidney-Qj Deficiency model. The normal group were injected equivalent normal saline, After two weeks, the model rats curled up in a state and were lassitude,24h proteinuria was more than100mg,then the models were copied successfully. Random samplled twentyfour rats from the copied model rats and randomly dividing them into three groups:nephropathy group, low and high dose Ginsenoside Rgl treatment group. The general position was observated, weight was weighed, Twenty-four hours urine protein and renal function were measured the day before the rata were killed when the four weeks of treatments had been completed. Euzymelinked immunosorbent assay(ELISA) were performed to examine the protein expression levels of IL-4and TNF-a, respectively. The renal pathological and podocyte changes were evaluated.Results:1. The nephropathy group rats'general position was bad, there were no obvious differences during the four groups (P>0.05)2. The nephropathy group's urine protein on the twenty eighth day increased Obviously compared with the normal and treatment group (P<0.01)3. The nephropathy group's serum albumin decreased significantly,the cholesterol increased compared with the normal and treatment group (P<0.01).There was no obvious difference on albumin urea and creatinine during the four groups (P>0.05)4. Compared with the normal group,The blood concentration of IL-4,TNF-a rats with nephropathy and Kidney-Qj Deficiency increased (P<0.01). The blood concentration of IL-4in the treatment group was inferior to the nephropathy group(P<0.01),but higher than the control group(P<0.05). The Ginsenoside Rgl treatment groups'blood concentration of TNF-a decreased significantly than nephropathy group (P<0.01), and had no difference with nomal group (P>0.05)5.The electron microscope showed that the nephropathy group's foot process fusion was more obvious than treatment group's.Conclusions:1.Ginsenoside Rgl can improve the serum biochemical index, renal function and podocyte lesion.It can protect the nephridial tissue and repair kidney qi.2. The mechanism of Ginsenoside Rgl protecting the nephridial tissue concludes decreasing blood conclusion of IL-4and TNF-a.3.From the above conlusions it can be detected that the mechanism of Ginsenoside Rgl repairing kidney qi concludes regulating immune.