Expression And Clinical Significance Of Sox9,Osterix In Hereditary Multiple Exostoses

BackgroundHereditary Multiple Exostoses (HME) is the most common benign bone tumor in children, and the growth of it is cease after puberty. The imaging performance is the cartilage cap of chondroma emerges to the surface of bone, extraversion growth, and the number of exostosis is different in person. It often occurs in the metaphysis, and it is easy to damage the metaphysis for children and lead to deformity. The treatment of this disease is to removal exostoses which had already affected the quality of patients'life. The other parts of the exostosis were taken reservation measures. If the surgical resection is tanggung, it will relapse and needs to receive reoperation, even repeatedly surgery. In recent years, because of the rapid development of molecular biology techniques, the scientist study the disease from the macro research gradually turned to the microscopic field, and the research is more in-depth study of the pathogenesis. Nowaday in the process of the formation of the bone,there were many factors which had transcriptional control for the formation of the bone。Sox9is an important embryonic development-related genes. In the process of growth, the Sox9is very closlely relationship with differentiation of chondrocyte and the bone formation. Osterix is a transcription factor with zinc finger which was found in mice in2002by Nakashima, It relates to the osteoblast differentiation and bone formation, while it control a variety expression of osteogenic factors.ObjectiveTo study on the level of expression and correlation analysis with Sox9and Osterix in the exostoses and peripheral blood serum in HME patients. And discuss the function and the correlation among them in the pathogenesis mechanism of HME。Methods1. MaterialWe selected the20cases of HME patients who chose the surgical to removal exostosis in the orthopedics ward of the Third Affiliated Hospital of Zhengzhou University during October2009and September2011, as the experimental group, and selected the20cases of development dislocation of the hip who need to have Orthodontic treatment for surgery in the same period at hospital as the control group. The diagnosis of HME is the2nd edition of the bone tumors organization classification by WHO.2. MethodsFirst, we make the HE staining in the sections of paraffin tissue between the exostosis in HME and the cartilage in the control group, and record the morphological observation in the microscopic; Using the method of immunohistochemical SP to detecte the expression of Sox9and Osterix in the sections of paraffin tissue between the two groups; Using the method of ELISA to detecte the expression of Sox9and Osterix in the serum samples between the two groups.3. StatisticallySPSS17.0software is used for statistical analysis of data, all data is used by mean±standard (x±s). Comparison between the two mean used t test. The linear correlation analysis is used Pearson to determine the relationship of two factors correlation analysis. Statistically significant level was considered as "α=0.05"Results1. The cell morphology of the tissue of chondroma in the group of HMEWe found the mature chondrocytes at the junction of the exostosis and normal bone were arranged in columnar in the experimental group, and observed the significant calcification and ossification, and the direction and shape of trabecular distribution is irregular. We found the chondrocytes were mature, the volume increased, and observed the vacuolization and irregular columnar.2. The localization expression of Sox9, Osterix in the tissue of chondroma between the HME group and the control groupSox9and Osterix were seen varying degrees of positive expression in the tissue of chondroma in two groups. Sox9is expressed mainly in the cytoplasm of chondrocytes, and the color of the cytoplasm is brown. Osterix is expressed in the cytoplasm was brown or brownish yellow coloring, also a little expression in extracellular matrix. The levels of expression and the areas of positive staining in chondrocytes about Sox9and Osterix in HME were significantly higher than the control group.3. The comparison expression of Sox9and Osterix in chondroma between the HME group and the control group.The expression of Sox9in the tissue of chondroma is higher than the control group, and the difference between them has statistically significant (p <0.05).The expression of Osterix in the tissue of chondroma is higher than the control group, and the difference between them has statistically significant (p <0.05).4. The comparison expression of Sox9and Osterix in the serum of the HME group and the control group.The Sox9in the serum of HME in the experimental group is higher expression than the control group, the difference has statistically significant (p <0.05). The Osterix in the serum of HME in the experimental group is higher expression than the control group, the difference has statistically significant (p <0.05).5. The correlation analysis of the expression of Sox9and Osterix in the chondroma tissue and serum in the HME groupIn the HME group, the relationship of Sox9and Osterix which expressed in the chondroma has positive correlation(p <0.05);The relationship of Sox9and Osterix which expressed in the serum of HME has positive correlation(p <0.05);In the HME group, the expression of Sox9in the chondroma has positive correlation with the expression of Sox9which expressed in the serum of HME (p <0.05). The expression of Sox9in the chondroma has positive correlation with the expression of Osterix which expressed in the serum of HME (p <0.05).In the HME group, the expression of Osterix in the chondroma has positive correlation with the expression of Osterix which expressed in the serum of HME (p <0.05).The expression of Osterix in the chondroma has positive correlation with the expression of Sox9which expressed in the serum of HME (p <0.05).Conclusions1. It may be involved in the pathogenesis of HME that the high expresstion of Sox9and Osterix in the serum of peripheral blood and in the chondroma tissue.2. In the process of growth and development of the bone, Sox9and Osterix may play a key role in regulation, and the high expression may lead the abnormal growth of bone through induce and regulate the genes in vivo, but the specific mechanism needs to further study.