Expression Of Visfatin,Omentin And HMGB1 In Peripheral Blood And Umbilical Cord Blood Of Patients With Hypertensive Disorder Complicating Pregnancy

Background:hypertensive disorder complicating pregnancy (HDCP) is a pregnancy-specific disease and the pathogenesis has not been elucidated. Vascular endothelial damage and inflammatory injury has been considered as the key step between the pathological changes and the HDCP. A number of vasoactive factors are related to the endothelial injury and play an important role in predicting changes in HDCP. Visfatin and omentin are adipocytokines discovered recently. The abnormal expression of visfatin is highly related to the obesity, type II diabetes and insulin resistance as well as other related diseases, which effects on vascular endothelial cells, leads to dysfunction. The less expression and activation of the omentin can induce microvascular permeability and lead to the increase of inflammatory cells. High mobility group protein B1 (HMGB1), one kind of nuclear non-histone proteins, can be released into the extracellular matrix and induce inflammatory response. The inflammatory cytokines plays an important role in the oxidative stress of HDCP. However, systematic investigation of visfatin, omentin and HMGB1 in the HDCP and cord blood serum has not been reported so far. In this study, the relationship between these cytokines, visfatin, omentin and HMGB1, and the HDCP will be addressed.Purpose:To investigate the expression of visfatin, omentin and HMGB1 in venous serum and umbilical cord blood of patients with HDCP.Methods:Total 90 cases of the HDCP diagnosed at birth of maternal pregnancy were collected from the People's Hospital of Tengzhou. These cases were divided into three groups:(1) 30 cases of the gestational hypertension, (2) 30 cases of the mild preeclampsia, (3) 30 cases of the severe preeclampsia, and 30 cases of the normal full-term pregnant women were included in the control group without statistical difference in gestational age and weeks. All cases employed here were the single fetus and the first production without the essential hypertension, cardiovascular diseases, acute or chronic infections, and autoimmune diseases. For each case, prior to delivery fasting, total 3 ml of venous blood sample was collected into the anticoagulation tube, and then centrifuged under 3000r/min for 10 minutes at 4℃. Subsequently, the supernatant samples were kept in-20℃freezer. Similarly,3 ml of theumbilical cord blood was collected immediately after the birth and processed as the above. The levels of visfatin, omentin and HMGB1 were analyzed with enzyme-linked immunosorbent assay (ELISA) Kits according to the manufacturer's protocol. ELISA plates were read using DNM-9606 Microplate Reader. Statistical analysis was carried out with SPSS 13.0 and One-way ANOVA software.Results:The visfatin,omentin and HMGB1 were detected in all groups. The serum contents of visfatin between patients and controls were significant difference (t=7.55, 10.05,10.97, P<0.05) as well as among these three HDCP groups (t=7.69,9.01,7.88, P<0.05). Similarly, the levels of omentin between patients and controls were significant difference (t=7.82,9.83,10.87, P<0.05), and also the significant differences were observed among these three patient groups (t=7.93,9.74,8.26, P<0.05). In addition, the levels of HMGB1 between patients and control group were significant difference (t=7.73,9.58,10.52, P<0.05) as well as these three HDCP groups (t=7.67,8.51,7.49, P<0.05). Furthermore, the positive relationship between visfatin and HMGB1 in HDCP was finded, whereas the negative correlations were observed among omentin and visfatin,HMGB1 in HDCP. Importantly, the levels of visfatin, omentin and HMGB1 in the umbilical cord blood were no significant differences among these three HDCP groups (P>0.05).Conclusion:Either the high expression of both visfatin and HMGB1 or the low expression of omentin in patients with HDCP reveals that the injury of vascular endothelium cells and visfatin play an important role in the development of injury. There are no significant differences detected in the levels of Visfatin, omentin, and HMGB1 in neonatal cord blood in each group with the hypertensive disorders in pregnancy, indicating that the HDCP can not cause the damage of fetal vascular endothelial. Moreover, visfatin, omentin, and HMGB1 are in high correlation in HDCP, suggesting that an important potential implication of these cytokines may be as inflammatory markers in HDCP.