Cancer Patients, Serum And Liver Cancer In Mice, Fluorescence Spectroscopy Study

Cancer is a challenge in the medical field. Diagnosis of incipient cancer is of great significance. With high sensitivity, high speed and relatively simple acquisition and processing of sample, fluorescence spectrum is an effective method in the analysis of material composition and molecular structure. The detection and analysis methods of fluorescence spectra into the tumor detection are of high hopes, then there are some difficulties to be solved.The serum fluorescence spectra of the healthy people, tumour patients, healthy mice and hepatoma mice are obtained experiment healthy ally. By analyzing the spectral characteristics and emission mechanism, the relationship between fluorescence peak intensity and peak frequency shift of different wavelengths are investigated. The findings demonstrate that the fluorescence spectral are excited by wavelength ultraviolet light of 260～280 nm, different degrees of blue shift are produced in the serum fluorescence spectrum peak location of tumour patients compared to healthy people. The same to the hepatoma mice compared to that of the healthy mice. When the wavelength of exciting light ranges from 400～440 nm, anomaly structure characteristics are discovered in the fluorescence spectra ranging from 620～630 nm of the tomour patients.Serum fluorescence spectrum characteristics of healthy people, tumour patients, healthy mice and hepatoma mice are researched by derivative spectra analysis. The result reflects that for 260 nm laser excited case, second derivative spectra of both human serum and mice serum show four minimums, but the peak locations of the minimums are different. Compared to healthy people, the peak location of second derivative spectra of tumour patients serum blue shifts are about 2-5 nm. Compared to healthy mice, the minimum peak position of second derivative spectra of hepatoma mice appear blue shifts about 4～9 nm. The relative intensity of the second derivative spectra minimum of the tumour patients are obviously superior to the control group.Serum fluorescence spectra of healthy people and tumour patients are studied by gauss decomposition. The difference between the parameter combination states such as half-width and relative intensity of the Gaussian basis between the tomour group and the control group is also discussed. The findings indicate that for 260 nm laser excited case, peak strength of Gaussian basis at 325 nm of tumour patients and the relative strength of the original spectrum are obviously higher than the control group. However, the relative strength of the other basis peaks tends to decrease. Analyses show that the intensity increase of fluorescence groups at 325 nm leads to the change of the entire spectral lines, which causes a blue shift of the fluorescence spectrum peak position.The research also indicates that for 405 nm laser excited case, the comparison between basis peak intensity near 620 nm and the strength of original serum spectra of the tumour group are higher than the control group. The research result is in conformity with the report in the current document that the content of protoporphyrin for tumour patients are higher than the healthy people.This research will contribute to the application of fluorescence spectroscopic methods into the early detection of cancer.