Liver Cancer Serum Fluorescence Spectrum Of The Gaussian Element Function

The research of incipient cancer diagnosis is an important and valuable subject. Fluorescence spectroscopy is sensitive and needs only a few of experimental samples. Thereby, the fluorescence spectroscopy is a diagnosis method for incipient cancer with great development potential.The relationship between tumor and signal intensity variation of protoporphyrin in serum fluorescence spectrum is attracting great attention. However, this signal is difficult to resolve and identify with respect to its weak intensity, which becomes the difficult problem in the research field.In this study, the serum fluorescence spectrums of tumor mice and normal mice were compared by using Gaussian basis function. The results suggested that the peak position (center wavelength), relative half-width and relative intensity of Gaussian basis functions for their serum fluorescence spectrums were quite different.For mice which were inoculated with liver cancer, the serum samples were collected and the fluorescence spectrums were detected for fifteen days successively. The excited optical wavelength was 405nm and 340nm respectively. Furthermore, the second derivative curve of the detected fluorescence spectrum was obtained and analyzed. After that, multi-peak Gaussian fitting functions were obtained according to the quantity and position of the minimum of the second derivative curve. Particularly, some peak positions (center wavelength), half-width and relative intensity of the Gaussian basis functions were compared and analyzed.In the 405nm light excitated case, the result showed that the relative half-width (the half-width ratio of 590nm and 573nm Gaussian basis functions) which represented zinc protoporphyrin (center wavelength 590nm) was increasing with liver cancer inoculated days. And the deep mechanism and theory needs to be studied in the future work. Besides, the relative intensity I573/I555 and I590/I555 of tumor mice group were higher than that of normal mice group.In the 405nm light excitated case, it can be concluded that the ratio of half-width of 490nm and 435nm basis functions, and the ratio of half-width of 495nm and 461nm basis functions were decreasing with liver cancer inoculated days.This research will provide theory and experimental basis for related investigations such as the mechanism of light-biology interaction, photobiology effect. The obtained results will also present valuable information for the application of new development fluorescence spectrum technology in incipient cancer diagnosis.