Study On The Diabetes And Bladder Cancer Diagnosis By Fluorescence Spectroscopy Of Urine

Spectroscopy analysis technique is efficient,rapid,no reagents and can be run at low cost,it has become an important method in clinic analyses field.Using the method of spectroscopy to study the early cancer will have a profound affection on the clinical medical analyze.Urine contains a wide variety of substances,the change of components in urine can not only reflect pathological change in urinary system and reproductive system,but also relate to a lot of whole body diseases.The purpose of this paper is to research the possibility of early diagnosis of diabetes and bladder cancer by fluorescence spectrum technology when the urine is used as sample.Using the urine excited by an Argon ion laser at wavelength of 488.0nm as sample,the fluorescence spectrums of sixteen normal persons,thirty-six diabetic patients and eleven bladder cancer patients were collected from 495nm to 640nm,and we had used the second derivative spectrum and wavelet transform spectrum to process data intensively.From the statistic results we could arrive at several conclusions.For diabetes,we found that there would be three minimum values in the second derivative spectrum of normal person if we divided the second derivative spectrum into four ranges,in contrast there were four minimum values in most second derivative spectrums of diabetes,the diabetic urine had one minimum value more than normal urine during the range from 550nm to 570nm.Whether the second derivative spectrum has minimum value during the range from 550nm to 570nm is used as a criterion,we can distinguish the diabetic urine from normal urine easily and precisely.For bladder cancer,using the second derivative spectrum and wavelet transform spectrum to process data,we found the conclusion was similar to the analysis of diabetes.In addition,from the average value of the light intensity ratio(I₄₉₅/ I₆₄₀) we found the diabetic patient was more than normal person,but bladder cancer was less than normal person.After consulting a lot of literature,we think the peak between 520nm and 550nm maybe come from riboflavin in the urine;and the peak between 600nm and 620nm maybe come from coproporphyrin;though we can't conclude where the peak between 520nm and 550nm derive from,we think it may be zinc protoporphyrin.At last,we don't know which substance form the peak between 550nm and 570nm,it is worthy of researching more details,it may be caused by the change of elements and concentration of urine.