Zun Shen Zhuang Gu Tang Affect Transformation Growth Factor Influence Of TGF-β₁ Expression In Bone Repair

Objective:Observation of traditional Chinese medicine zun shen zhuang gu tang in rabbit bone fracture repair mophorlogical changes and fracture end transformation growth factor (β1) expression TGF-β1 on the research of rabbit zun shen zhuang gu tang and the role of fracture repair.Methods:Select health New Zealand male rabbit only (30), which will be subjected to try any animal into two groups at random:A of the group, and group B as control group, each group of 15 only. Radial fracture defect caused by bilateral animal model, adaptive raise 1 week, and each irrigation experimental served zun shen zhuang gu tang (5.6 ml/kg/day) diluted properly stomach, twice a day filling group, irrigation clothing saline (5.6 ml/kg/day), every second. After 3 weeks (the use of air embolism law), two groups of death animal experimental animals are executed intraperitoneal injection before 50mg/kg for mark to tetracycline, first executed nine days before the second death for medicine, before 3 days to medicine, take the radial fracture end with bilateral centered about 1.2 cm long bone fixed then in 40% respectively,70%, 80%,95%,100% ethanol series dehydration, xylene transparent, methyl methacrylate penetration fixation, embedding, made with heavy hard slice machine specimen slice with fluorescent image analysis system, image analysis, organize morphological examination; Postoperative 1,2,3 weeks both groups were put to death, taking five rabbit radial fracture end with bilateral centered about 1.2 cm long bone fixation, demineralization and alcohol dehydration, transparent, paraffin embedding made 3u m plating, militiamen and immunohistochemical staining.Results:After 3 weeks of morphological index:bone tissue are wide and class bone surface, between the double marking tetracycline average distance and bone deposition rates of mineralization, compared with the control group was significant difference; Organization morphological observation:1 weeks, the control group in fibrous connections stage, mainly by fibroblasts with a small amount of inflammatory cells infiltrating osteoblast group formed, fibroblasts, remain relatively more small granulation tissue and cartilage, a little inflammatory cells infiltrating; 2 weeks, the control group which numerous chondrocytes and fibroblasts and cartilage tissue hyperplasia obvious, visible rare flat surface visible osteoblast, bone cells and the bony father some into such bone. The visible more chondrocytes and abundant fibrous tissue, cartilage cells more for mature fat large, fibrous tissue, closely spaced in bone surface occurs, the number of osteoblast more around a small cut ends new bone appear.3 weeks, the control group fracture end bone of the outer membrane disrupted trabeculae seen new bone, class is less, callus place outside fibrous to partly visible within the bony scab, callus more. The new bone trabeculae increased, arranging disorderly and osteogenesis process obvious, broken bone cells accidentally see, callus more for hard outside, callus callus in more, lots of new capillary zhang trabecular marrow. Immunohistochemical observations and image analysis:fracture experimental and control group after 1 week around the outer membrane and bone formation in the soft hematoma neutrophils, lymphocytes, monocytes and TGF stroma in a red dye, a weak positiveβ1 TGF-β1 between the two groups express no obvious difference; 2 weeks after the fracture in cartilage formation area visible cartilage cells and mature immature cartilage cells is strong positive dyeing, and mature chondrocytes shading the deepest, cartilage cells don't show color hypertrophy of group, also visible to cartilage cells have deep shading, but obviously shallow at the experimental group, there were significant differences between comparison group (P<0.05). Three weeks after the fractured bone cells, callus of osteoblast and callus surface cartilage cells of a strong positive expression within group, dyeing positive expression, there were significant differences between comparison group (P<0.05).Conclusion:1, tip zun shen zhuang gu tang to enhance distinguishes fracture repair TGF-β1 expression, can promote bone repair and osteogenesis role.2 and kidney strong bone soup of rabbit fracture healing repair process, the specific mechanism and clinical significance in further research needs to be.