Fgfr2 Gene Single Nucleotide Polymorphism And Breast Cancer Research

Objective:To ensure whether the single nucleotide polymorphisms in the second intron of the fibroblast growth factor receptor 2 (FGFR2) gene and the development of breast neoplasm in female population is related.Methods:The distribution of polymorphisms rs2912778, rs3135718 and rs11200014 in FGFR2 gene were analyzed by allele specific amplification (ASA) and agarose gel electrophoresis in 200 cases with breast neoplasm and 200 samples of normal controls. At the same time, PCR products were sequenced to verify the accuracy of the purpose strip.Results:Significant differences were found in the genotypes between the breast neoplasm group and the control group (P<0.01). No difference was found in allele frequency of rs2912778 (P>0.05) and rs11200014 (P>0.05) in the case group and the control group, while significant difference was found in rs3135718 (P<0.01). There was a non-significant difference between the distributions of wild-type and variant-type in three mutation sites, with respect to the tumor size, histological grade and lymph node metastasis (P>0.05)Conclusions:The single nucleotide polymorphism in the second intron of the FGFR2 is probably associated with the incidence of breast neoplasm in Chinese women.